遗传工程菌Fhhh降解精对苯二甲酸与mnp基因表达  

Degradation of purified terephthalic acid and expression of mnp gene for GEM Fhhh

作　　者：程树培[1] 陈亮[1] 严俊[1] 郝春博[1] 李维新[1] 顾继东[2] 程攻[3] 陈兰明[3] 

机构地区：[1]南京大学污染控制与资源化研究国家重点实验室环境学院,南京210093 [2]香港大学生态与生物多样性系生态毒理实验室 [3]南京大学生物科学与技术系

出　　处：《环境科学学报》2002年第1期1-5,共5页Acta Scientiae Circumstantiae

基　　金：江苏省科委自然科学基金 (BK- 990 33) ;香港中国环保工业有限公司资助

摘　　要：跨界融合构建的遗传工程菌Fhhh及其亲株黄孢显毛平革菌 (PC) ,降解精对苯二甲酸的比降解率受到Mn2 + 、酒石酸铵、H2 O2 、pH共 4因素的影响 .除pH值外 ,其余 3个因素影响 2菌株比降解率大小的排序完全一致 .pH值对PC菌的影响排序处于第 3位 ,对Fhhh则处于第 1位 .Fhhh比降解率比PC高出 11 11% ;mnp基因表达的锰过氧化物酶 (MnP)比活力水平比PC高出 15 2 2 % .降解精对苯二甲酸 4因素优化水平 ,也是mnp基因表达的优化条件 .比降解率与MnP的比活力水平之间有显著或极显著正相关性 (r>r0 .0 5( 4) ,r >r0 .0 1( 4) ) .The levels of the specific degradation rate (SDR) for the genetically engineered microorganism (GEM) Fhhh from inter\|kingdom protoplast fusion and the parental strain \%Phanerochaete chrysosporium\% (PC) in degradation of purified terephthalic acid (PTA) were affected by (NH\-4)\-2C\-4H\-4O\-6,H\-2O\-2,Mn\+\{2+\},and pH value.Except pH value,the other 3 factors affecting the SDR levels had a same order for both strains.The pH value in the order was at the first site for Fhhh,and that for PC at the third site.At the optimal levels,the SDR value of Fhhh was 11\^11% higher than that of PC;the value of manganese peroxidase specific activity (MnP\|SA) of Fhhh was 15\^22% higher than that of PC.There was a positive relationship between SDR and MnP\|SA significantly.The optimal levels of 4 factors for SDR also were the optimal levels for mnp gene expression.The technique of the mnp gene expression control would be useful to treat PTA wastewater.

关 键 词：跨界隔合子 黄孢显毛平革菌 精对苯二甲酸 比降解率 相关因素 锰过氧化物酶 基因表达 FHHH 废水处理 遗传工程菌 降解 

分 类 号：X172[环境科学与工程—环境科学] X703.5