肿瘤组织中免疫球蛋白基因SNC73的表达及其临床意义  

Expression of a novel immunoglobulin gene SNC73 in human cancer and its significance

作　　者：胡建斌[1] 邓甬川[1] 郑树[1] 

机构地区：[1]浙江大学肿瘤研究所

出　　处：《中华肿瘤杂志》2002年第1期38-40,共3页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目 ( 30 0 70 832 ) ;国家"863"高科技研究发展计划基金资助项目 ( 10 2 10 0 10 7)

摘　　要：目的　探讨免疫球蛋白基因SNC73在不同恶性肿瘤组织及其自身对照正常组织中的表达及临床意义。方法　采用逆转录聚合酶链反应酶联免疫吸附 (RT PCR ELISA)法 ,检测SNC73基因在 90例恶性肿瘤 (大肠癌、胃癌、乳腺癌、肺癌、肝癌 )及其相对应的对照正常组织中的表达 ,并对SNC73表达与大肠癌患者的性别、年龄、发病部位、分化程度、侵袭深度和转移等临床病理特征的相关性进行分析。结果　SNC73基因在正常黏膜及大肠癌表达量的比值为 7.134 ,差异有显著性 (P <0 .0 1)。胃癌、乳腺癌、肺癌和肝癌组织的SNC73表达量与自身对照正常组织比较 ,差异无显著性(P >0 .0 5 )。结论　SNC73的表达下调是大肠癌相对特异的现象 ,提示其可能为大肠癌发生发展中新的肿瘤标记 ,其与大肠癌发生发展的相关性 ,值得进一步研究。Objective To investigate the expression of a new immunoglobulin gene SNC73 in malignant tumor and normal tissue and its significance.Methods Expression level of SNC73 in tumors and normal tissues was determined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay (RT-PCR-ELISA) in 90 malignant tumors, including colorectal cancer, gastric cancer, breast cancer, lung cancer and liver cancer. Analysis on relation of SNC73 expression with age, sex, site, differentiation grade, depth of invasion and metastasis of colorectal cancer was made to assess the clinical significance of SNC73. Results Mean ratio of SNC73 expression level in normal mucosa and colorectal cancer tissue was 7.134 ( P <0.01). Expression of SNC73 showed no significant difference among gastric cancer, breast cancer, lung cancer or liver cancer as compared with the control normal tissues ( P >0.05). Conclusion Down-regulation of SNC73 expression is a relatively specific phenomenon in colorectal cancer for which development SNC73 may be a potential genetic marker. The study on relationship of SNC73 expression with development of colorectal cancer is promising.

关 键 词：结直肠肿瘤 免疫球蛋白基因SNC73 基因表达 聚合酶链反应 酶联免疫吸附测定 临床意义 

分 类 号：R730.2[医药卫生—肿瘤]