端粒酶反义核酸对BEL-7402肝癌细胞生长和活性的影响  

Inhibitory Effects of Antisense Oligonucleotides against Human Telomerase RNA on Hepatocarcinoma Cell BEL-7402

作　　者：曹英林[1] 赵丽[1] 孙汶生[1] 薛伟华[2] 

机构地区：[1]山东大学医学院免疫研究室,250012 [2]山东大学药学院生化制药教研室,250012

出　　处：《实用癌症杂志》2002年第1期13-15,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金资助 (批准号 30 0 70 341 )

摘　　要：目的　研究人类端粒酶RNA(hTR )基因的反义寡核苷酸 (AS ODN )对BEL 740 2肝癌细胞生长和活性的影响。方法　将AS ODN作用于BEL 740 2细胞 ,观察细胞生长状况 ,PCR ELISA法检测端粒酶活性 ,流式细胞仪和细胞DNA电泳观察细胞的凋亡情况。结果　AS ODN能抑制BEL 740 2细胞的生长 ,降低其端粒酶活性并诱导细胞凋亡。结论　针对hTR的ASObjective To investigate the effects of antisense oligonucleotides(AS ODN) against human telomerase RNA(hTR)on BEL 7402 hepatocarcinoma cell.Methods After incubation with the AS ODN,growth inhibition of BEL 7402 cell was observed,telomerase activity were measured by PCR ELISA and apoptosis by flow cytometry and DNA fragment analysis.Results Cell growth and telomerase activity were ihhibited by AS ODN.Induction of apoptosis were also observed.Conclusion The AS ODN of hTR have potential therapeutic effects on hepatocarcinoma.

关 键 词：端粒酶 反义核酸 肝癌细胞 BEL-7402 肝癌 

分 类 号：R735.7[医药卫生—肿瘤]