大鼠局灶性脑缺血半暗带葡萄糖转运子3 mRNA的表达  

Expression of glucose transporter-3 mRNA in rats' focal cerebral ischemic penumbra

作　　者：陈元新[1] 林祥通 符荣[2] 

机构地区：[1]上海复旦大学附属华山医院核医学科,200040 [2]复旦大学附属华山医院神经外科

出　　处：《临床神经病学杂志》2002年第1期9-11,共3页Journal of Clinical Neurology

基　　金：核医学国家重点实验室资助课题 ( 2 0 0 0 -0 6 )

摘　　要：目的　研究大鼠局灶性脑缺血不同缺血时间皮质半暗带和中心区葡萄糖转运子 3(GL UT3 )转录水平的表达规律。方法　用插线法建立大鼠局灶性脑缺血模型 ,剥取缺血半暗带及中心区皮质组织 ,采用逆转录 -聚合酶链反应 (RT- PCR) ,测定 GL U T3 m RNA水平的变化。结果　缺血半暗带 GL U T3 m RNA在缺血 3小时升高 ,2 4小时达到高峰 ,96小时后基本恢复正常。缺血中心区 GL U T3 m RNA在缺血后 3小时有一短暂的升高 ,随后迅速下降呈低水平表达。结论　 GL UT3 在缺血半暗带的表达上调 ,有可能是机体对缺血损伤的保护性反应。Objective To investigate the expression of glucose transporter 3(GLUT 3) transcription level at different ischemic time in rats' focal cerebral ischemic penumbra and core of cortices.Methods Focal ischemic models of middle cerebral artery occlusion (MCAO) in rats were made by inserting nylon surgical thread. Brain samples were harvested from ischemic penumbra and core of cortices. The change of GLUT 3 mRNA was assessed by RT PCR.Results GLUT 3 mRNA in the penumbra regions slightly increased at 3 h after ischemia, and reached a peak at 24 h, in 96h following ischemia it came back to the normal level. As for ischemic core regions, GLUT 3 mRNA had a short time increase at 3 h after ischemia, and then rapidly decrease to the low level. Conclusion GLUT 3 mRNA expression was notably up regulated in the penumbra regions after focal cerebral ischemia,it may be a protective reaction against ischemic injury.

关 键 词：脑缺血 半暗带 葡萄糖转运子3 转录 表达 

分 类 号：R743[医药卫生—神经病学与精神病学]