大鼠中缝背核内一氧化氮合酶阳性神经元向中央杏仁核的投射  

Nitric Oxide Synthase Positive Neurons of the Dorsal Raphe Nucleus Projecting to the Central Amygdalagy Nucleus in Rat

作　　者：肖明[1] 丁炯[1] 吴凌霞[1] 左国平[1] 颜艳[1] 

机构地区：[1]南京医科大学人体解剖学与组织胚胎学系,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2002年第2期132-134,I001,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：南京医科大学科技发展基金资助项目(NY99006)

摘　　要：目的：观察大鼠中缝背核（ＤＲ）内一氧化氮合酶（ＮＯＳ）阳性神经元向中央杏仁核（ＣｅＡ）的投射，为进一步阐明一氧化氮（ＮＯ）参与情绪、疼痛、内脏心血管活动的中枢调节机制提供形态学资料。方法：用黄递酶（ＮＡＤＰＨ－ｄ）组织化学染色结合辣根过氧化酶（ＨＲＰ）逆行追踪技术，观察大鼠ＤＲ内向ＣｅＡ投射的ＮＯＳ阳性神经元的分布与形态。结果：ＤＲ内有一定数目的ＮＯＳ／ＨＲＰ双标阳性神经元，主要分布在外侧部与背正中部。结论：ＤＲ内有部分ＮＯＳ阳性神经元向ＣｅＡ投射，提示ＮＯ在ＤＲ至ＣｅＡ上行感觉传导通路中起重要的神经递质作用。Objective:To provide the morphological data for furth er understanding the role of nitric oxide(NO) in central modulation of pain, moo d and visceral and cardiovascular activity, we studied nitric oxide synthase(NOS ) positive neurons of the dorsal raphe nucleus(DR) projecting to the central amy gdalagy nucleus(CeA) in rat. Methods: Localization of DR NOS positive neurons projecting to CeA was evaluated by nicotinamide adenine dinucleotide pho sphate naloxone diaphorase histochemical technique combining with horseradish pe roxidase(HRP) as a retrograde tracer. Results: A few NOS/HRP double-l abeled neurons were found in the DR, it majority was distributed at the lateral and the dorsal median part. Conclusion: The DR has some NOS positive neurons projecting to the CeA. It suggests that NO may act as an important neuro transmitter in the ascending sensory pathways of the DR.

关 键 词：中缝背核 中央杏仁核 一氧化氮合酶 投射 大鼠 阳性神经元 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]