大鼠胃黏膜癌变过程细胞凋亡及相关癌基因bcl-2、c-myc的动态表达  

Apoptosis and expression of oncogene bcl-2,c-myc in carcinomatous change of gastric mucosa in Wistar rats

作　　者：林庚金[1] 范钰[1] 钱立平[1] 许祖德[2] 李华[2] 

机构地区：[1]复旦大学附属华山医院消化科,200040 [2]复旦大学基础医学院病理教研室

出　　处：《上海医学》2001年第7期417-419,共3页Shanghai Medical Journal

摘　　要：目的　探讨大鼠胃黏膜癌变发生发展过程中细胞凋亡及其相关基因的关系。方法　采用MN NG、10 %NaCl及酒精灌胃建立了大鼠胃黏膜癌变模型 ,设立正常空白组、模型组 ,分别用TUNEL、原位杂交技术及免疫组化大鼠检测胃黏膜细胞凋亡指数、bcl 2、c myc基因表达。 结果　本造模方法成功率较高。从正常胃黏膜到胃癌 ,细胞凋亡指数逐渐下降 ,bcl 2、c myc基因表达逐渐增高。大鼠胃黏膜细胞凋亡受到抑制与bcl 2、c myc基因高表达具有高度的一致性。 结论　细胞凋亡失控是胃黏膜癌变发生发展的机制之一 ,bcl 2、c myc基因可能参与了对大鼠胃黏膜癌变细胞凋亡的调控。Objective To study the correlation of apoptosistran and oncogene. Methods We studied expression of bcl 2,c myc gene and apoptosis using In situ hybridization , TUNEL in rats in two groups: 1. Normal control,2. Model control by MNNG,aocohol and 10% sodium chloride. Results The rate of model is high. With the development of gastric cancer ,the exepression of bcl 2/c myc gene and supression of apoptosis were increased by degrees,and, the former has an important correlation with the latter. Conclusion Conclusions Apoptosis has play an immpormant role in carcinomatous change in gastric mucosa,and bcl 2 、c myc were regulate apoptosis

关 键 词：胃肿瘤 细胞凋亡 BCL-2 C-MYC 胃粘膜癌变 大鼠 

分 类 号：R735.2[医药卫生—肿瘤]