荧光聚合酶链反应用于阴道炎性病病原体检测  

DETECTION OF STD PATHOGENS IN VAGINITIS BY FLUORESCENT PCR TECHNIQUE

作　　者：邬玲仟[1] 李小红[1] 蔡珠妇 黄志峰[2] 杨欣源[3] 

机构地区：[1]粤北人民医院遗传中心,广东省韶关市512026 [2]粤北人民医院产科,广东省韶关市512026 [3]中山医科大学达安基因诊断中心

出　　处：《中国煤炭工业医学杂志》2001年第12期964-965,共2页Chinese Journal of Coal Industry Medicine

摘　　要：目的　了解阴道炎患者生殖道性病病原体NGH、CT、Uu、HPV6 ,1 1 流行情况。方法　将PCR技术与荧光素标记探针相结合 (FQ -PCR) ,对阴道炎患者的宫颈柱状上皮进行NGH、CT、Uu、HPV6 ,1 1 定量检测。结果　阴道炎患者中四种病原体总感染率95 .70 % ,阳性率依次为Uu(70 .0 0 % ) >CT(61 .30 % ) >NGH(1 4 .0 0 % ) >HPV6 ,1 1 (1 0 .30 % )。病原体混合感染率 (51 .30 % ) ,以解尿支原体并发其他病原体感染率最高 (45 .30 % ) ,其次是沙眼衣原体 (44.30 % ) ,淋球菌 (9.0 0 % ) ,尖锐湿疣 (5 .70 % ) ,病例中 2 0～ 39岁患者感染NGH、CT、Uu、明显多于其他年龄组。结论　对阴道炎患者医生应尽可能做各种性病病原体检测 ,以明确诊断 ,及时治疗。Objective To investigate the epidemic state of genital sexually transmitted disease (STD) pathogens like NGH, CT, Uu and HPV6,11 in the patients with vaginitis. Methods In combination of PCR technique with fluorogenic (FQ-PCR), NGH, CT, Uu and HPV6,11 were quantitatively detected. Results The total infection rate of the four pathogens in the vaginitis patients was 95.70%, and the positive rates were Uu (70.00%)> CT (61.30%)> NGH (14.00%) >HPV6,11 (10.30%) in turn. 51.30% of the patients showed complicated infection of these pathogens, among which the infection rate of HGH combined with others was 45.30%, and then CT (44.30%), Uu (9.00%), HPV6,11 (5.70%) in the decreasing order. NGH, CT and Uu infection incidences in the patients of age-group 20-39 were significantly higher than in those of other age- groups. Conclusion For accurate diagnosis and prompt treatment for the patients with symptoms of vaginitis, more attention should be paid by detecting as many varieties of STD pathogens as possible.

关 键 词：阴道炎 荧光聚合酶链反应 性病病原体 

分 类 号：R711.31[医药卫生—妇产科学]