奥曲肽辅助肝动脉结扎治疗大鼠肝内移植瘤的初步观察  

Effects of Hepatic Arterial Ligation Combined with Octreotide for Transplanted Cancer in Rat Liver

作　　者：郭伟剑[1] 白永瑞[2] 庄军燕[1] 李杰[1] 张力[3] 张广慧[3] 严惠芳[4] 陆宏祺[4] 

机构地区：[1]上海第二医科大学附属新华医院肿瘤中心肿瘤科,上海200092 [2]上海第二医科大学附属新华医院肿瘤中心放疗科,上海200092 [3]上海第二医科大学附属新华医院肿瘤中心检验科,上海200092 [4]上海医药工业研究院肿瘤药理室,上海200437

出　　处：《复旦学报（医学版）》2001年第6期534-536,共3页Fudan University Journal of Medical Sciences

摘　　要：目的　观察奥曲肽辅助肝动脉结扎 (HAL)治疗大鼠肝内移植瘤的效果。方法　采用大鼠肝内移植Walker 2 5 6肿瘤模型 ,分为对照组、HAL组、HAL +奥曲肽治疗组。观察治疗后肿瘤体积改变与肿瘤生长抑制率 ,Hoechst 33342标记法检测肿瘤组织血供 (标记细胞数代表血供情况 ) ,检测ALT、AST观察HAL、奥曲肽对肝功能的影响。结果　对照组、HAL组、HAL +奥曲肽治疗组肿瘤体积分别为 (0 .10 3± 0 .0 4 3)cm3 、(0 .0 30± 0 .0 18)cm3 、(0 .0 16± 0 .0 0 5 )cm3 ,后 2组肿瘤体积明显小于对照组 (P <0 .0 1) ,肿瘤抑制率分别为70 .8%、84 .5 %。HAL +奥曲肽治疗组肿瘤体积明显小于HAL组 (P <0 .0 5 )。对照组、HAL组、HAL +奥曲肽组Hoechst 33342标记细胞数分别为 36 9.7± 30 .2、344 .1± 2 6 .0、32 3.2± 4 0 .4 ,HAL +奥曲肽组标记细胞数明显少于对照组 ,提示其血供明显减少。HAL及奥曲肽对肝功能无明显影响。结论　奥曲肽能明显提高肝动脉结扎治疗大鼠肝内移植瘤的效果 ,减少动脉阻断后的肿瘤血供可能为主要机制之一。Purpose: To investigate the effects of hepatic arterial ligation(HAL) combined with octreotide for transplanted cancer in rat liver. Methods: Walker 256 carcinosarcoma was transplanted into rat liver to establish a liver cancer model. Rats bearing tumor were divided into three groups: control group, HAL group, and HAL plus octreotide group. Changes of tumor volume, tumor growth inhibitory rate, and liver function after therapy were evaluated. Hoechst 33342 labeling assay was used to analyze the blood perfusion of tumor. Results: The mean tumor volume in control group, HAL group, and HAL plus octreotide group was (0. 103 ± 0.043)cm3, (0.030 ± 0.018) cm 3, and (0. 016 ± 0.005) cm3, respectively. The tumor volumes in the latter two groups were smaller than that in control group (P<0.01), and the tumor growth inhibitory rates were 70.8% and 84.5%, respectively. Compared with HAL group, the tumor volume in HAL plus octreotide group decreased significantly(P<0.05). The numbers of Hoechst 33342 labeled cells in control group, HAL group, and HAL plus octreotide group were 369.7 ± 30.2, 344.1 ± 26.0 and 323.2 ± 40.4, respectively. The number in HAL combined with octreotide group decreased significantly compared with that in control group (P<0.05), suggesting that the blood perfusion of tumor in HAL plus octreotide group decreased significantly. There was no obvious influence of HAL and octreotide on liver function. Conclusions: Octreotide can promote the effects of HAL for transplanted cancer in rat liver. Decreasing the blood perfusion of tumor after hepatic arterial blockage may be one of it's major mechanisms.

关 键 词：肝肿瘤 肝动脉结扎 奥曲肽 治疗 

分 类 号：R735.7[医药卫生—肿瘤] R730.58[医药卫生—临床医学]