乳腺癌中细胞周期素D1基因扩增和过表达及其与c-erbB-2的关系  

Amplification and overexpression of cyclin D1 gene in breast cancer and their relations to c-erbB-2 protein

作　　者：吴强[1] 张瑰红[1] 饶慧蓉[1] 杨枫[1] 官伟宁[2] 王劲[3] 

机构地区：[1]安徽医科大学病理学教研室,合肥230032 [2]安徽医科大学附属医院肿瘤科,合肥230022 [3]安徽医科大学附属医院普外科,合肥230022

出　　处：《安徽医科大学学报》2001年第6期420-423,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教委自然科学基金 (编号 :98jl0 6 1);国家科技攻关地方重大项目 (编号 :99 D0 5 0 )

摘　　要：目的　探讨细胞周期素D1基因和表达的改变在乳腺癌中的作用及其与c erbB 2表达的关系。方法　采用原位分子杂交和免疫组织化学技术检测 5 9例乳腺癌组织细胞周期素D1基因扩增、mRNA及其蛋白和c erbB 2的表达。结果　 2 7例 (4 5 8% )细胞周期素D1蛋白过表达 ,其过表达与腋淋巴结转移相关 (P <0 0 5 )。细胞周期素D1DNA扩增、mRNA高表达和蛋白过表达三者基本一致 ,与c erbB 2过表达无相关。结论　细胞周期素D1蛋白过表达在乳腺癌频发 ,其基因扩增和转录增加是其重要机制之一。细胞周期素D1和c erbB 2在乳腺癌中各自发挥独立作用 。Objective To investigate the role of the alteration of cyclin D1 gene and its expression in human breast cancer and their relations to c erbB 2 oncoprotein. Methods Cyclin D1 DNA amplification and its mRNA and protein overexpresion in 59 cases of breast cancer tissues were detected by in situ hybridization and immunohictochemical staining. C erbB 2 oncoprotein expression was detected by immunohictochemical technique, too. Result Cyclin D1 was overexpressed in 27 cases of breast cancer (45 8%), which was significantly related to lymphnode involvement ( P <0 05). Coherence was found between cyclin D1 gene amplification, its mRNA and protein overexpression. Cyclin D1 overexpression was not associated with c erbB 2 positivity. Conclusion There are frequent alterations of cyclin D1 in human breast cancer. One of the mechanisms of cyclin D1 overexpression is its DNA amplification. Cyclin D1 and c erbB 2 may play important roles independently in the development of breast cancer through different pathways. Simultaneous detection of the two proteins is helpful to evaluate prognosis of the patients.

关 键 词：乳腺肿瘤 病理学 细胞周期蛋白D1 基因 erbB-2免疫组织化学 原位分子杂交 

分 类 号：R737.9[医药卫生—肿瘤]