梅花鹿茸和马鹿茸多肽化学性质及生物活性比较  

A Comparison of Chemical Composition and Bioactivity of Polypeptides from Velvet Antlers of Cervus nippon Temminck and Cervus elaphus Linnaeus

作　　者：周秋丽[1] 刘永强[2] 王颖[2] 郭颖洁[2] 王本祥[2] 

机构地区：[1]白求恩医科大学生物工程研究所,吉林长春130021 [2]长春中医学院附属医院新药研究中心,吉林长春130021

出　　处：《中国中药杂志》2001年第10期699-702,共4页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金资助项目 ( 39870 92 9)

摘　　要：目的 :比较梅花鹿茸多肽和马鹿茸多肽化学组成及生物活性异同。方法 :用相同工艺提取梅花鹿茸和马鹿茸多肽组分 ;采用电泳和质谱等分离分析手段比较多肽组分的化学性质 ;用 [3H]TdR参入细胞DNA比较两种鹿茸多肽的促细胞增殖活性。结果 :梅花鹿茸和马鹿茸多肽组分的电泳图谱和质谱有明显差异 ,而中国的东北马鹿茸和新西兰马鹿茸多肽的图谱十分相近 ;梅花鹿茸和马鹿茸多肽对软骨细胞和表皮细胞分裂都有促进作用 ,但马鹿茸多肽对表皮细胞的增殖作用比梅花鹿茸多肽强。结论Objective: To compare the chemical composition and bioactivity of polypeptides(PPs) isolated from velvet antlers of sika deer ( Cervus nippon Temminck) and red deer ( Cervus elaphus Linnaeus).Method: The two kind of polypeptides were isolated from the above mentioned velvet antlers with same technology. The chemical composition was determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS PAGE) and MALDI TOF mass spectrometry. Stimulant activity of cells proliferation was measured by TdR incorporation into DNA. Result: The graphs of SDS PAGE and MALDI TOF MS of velvet antler polypeptides (VAPPs) from Chinese and New zealand red deer were very similar, but there were obvious difference in respect of graph between sika deer and red deer. VAPPs 25～50 mg· L -1 showed marked proliferation promoting activity for rabbit costed chondrocytes, either sika deer or red deer. However, the activity of sika deer VAPPs 12.5 mg·L -1 for epidermal cells was weaker than that of red deer (12.5 mg·L -1 ). Conclution: The chemical property and bioactivity of VAPPs from sika deer and red deer are significantly different.

关 键 词：梅花鹿 马鹿 鹿茸 多肽 软骨细胞 表皮细胞 化学性质 生物活性 比较 

分 类 号：R284.1[医药卫生—中药学]