胃癌活检和腹水标本p53基因序列变化的研究  

Sequence Alteration of p53 Gene in the Biospical Tissues and Ascites of Patients with Gastric Cancer

作　　者：戴正萍[1] 顾其华[1] 廖志丹[1] 叶爱慧[1] 

机构地区：[1]南华大学附属怀化医院,湖南怀化418000

出　　处：《南华大学学报（医学版）》2001年第5期453-454,464,共3页Journal of Nanhua University(Medical Edition)

摘　　要：目的　研究胃癌的发生、发展及预后与p5 3基因突变的关系。方法　用多聚酶链反应和DNA双链直接测序方法进行p5 3基因外显子 5、6、7、8序列分析 ,共检测 33例胃癌、17例胃粘膜非典型增生、31例胃癌术后并转移性腹水标本。结果　发现 9例胃癌和 7例胃癌术后并发腹水标本存在p5 3基因碱基突变 ,突变率分别为 2 7.2 7%和 2 2 .5 8% ,且在 7例腹水标本中发生p5 3基因突变的患者原病理切片组织中发现了相应的突变。17例胃粘膜非典型增生组织中亦发现 1例p5 3基因突变。结论　胃癌与p5 3基因突变密切相关 ,p5 3基因突变是胃癌较早期的生物学行为。Objective: To study the action of p53 gene in the genesis, development and prognosis of gastric cancer. Methods: Polymerase chain reaction (PCR) and double-strand DNA sequencing were applied to analyse exons 5, 6, 7, 8 of p53 gene in 33 cases of gastric cancer,17 cases of gastric mucosal atypical hyperplasia and 31 cases of peritoneal effusion from patients with gastric cancer. Results:27.27%(9/13) cases of gastric cancer, 22.58%(7/31) cases of peritoneal effusion and 5.88%(1/17) case of atypical hyperplasia showed p53 gene mutations respectively.The mutations in peritoneal effusion were just the same as that in previous embeded tissues. Conclusion: There is a close relation between gastric cancer and p53 gene mutation which may be a early biological behaviour in gastric cancer genesis.

关 键 词：胃癌 P53基因 测序 活检 腹水 

分 类 号：R735.202[医药卫生—肿瘤] R730.231.3[医药卫生—临床医学]