生长激素受体mRNA在肝细胞癌和癌旁肝硬化组织中的表达  

Expression of Growth Hormone Recept or mRNA in Hepatocellular Carcinoma and Matched Para-Cancer Cirrhotic Liver Tissue

作　　者：王洪涛[1] 王捷[1] 区庆嘉[1] 刘晓平[1] 陈双[1] 

机构地区：[1]中山大学孙逸仙纪念医院肝胆外科,广州广东510120

出　　处：《癌症》2002年第2期146-148,共3页Chinese Journal of Cancer

基　　金：广东省自然科学基金(编号:984213);原中山医科大学211工程重点学科建设基金(编号:F000099075)

摘　　要：背景与目的:正常肝组织富含生长激素受体mRNA,但在肝细胞癌、癌旁肝硬化组织中生长激素受体mRNA的表达情况不详,因此,本文旨在探讨肝癌组织、癌旁肝硬化组织中生长激素受体mRNA的表达情况。方法:采用逆转录-聚合酶链反应方法检测37例肝癌患者肝癌组织、癌旁肝硬化组织中生长激素受体mRNA的表达。结果:肝癌组织生长激素受体mRNA表达率(30/37,81.0%)较癌旁肝硬化组织的表达率(32/32,100%)低(P<0.05),未分化肝癌的表达率(1/4,20.0%)更低(P<0.5);癌旁肝硬化组织的生长激素受体mRNA的表达量犤积分光密度比值(riOD)为(30.77±8.24)%,n=32犦较正常对照肝组织的表达量犤riOD为(44.93±6.25)%,n=5犦少(P<0.05),重度肝硬化的表达量犤riOD为(21.90±4.72)%,n=8犦更少(P<0.05)。结论:生长激素受体mRNA的表达下调与肝癌的分化程度、癌旁肝硬化的严重程度有关。Background &Objective:The growth hormone receptor mRNA is a bundant in normal liver tissue,but i ts expression was not studied in detail in the tissue of liver cancer or para-cancer cirrhotic liver.This study w as designed to investigate the expression of growt h hormone receptor mRNA in hepatocellular carcinoma and para-cancer cir rhotic liver tissue.Methods:The expression of growth hormone rec eptor mRNA was detected in 37specime ns of hepatocellular carcinoma and their para-cancer liv er tissue by RT-PCR.Results:The expression rate of growth hormon e receptor mRNA in hepatocellular cancerous tissue(30/37,81%)was significantly lower than that in cirrhotic liver tissue(32/32,100%)(P<0.05).The expression rate in undifferent iated hepatocellular carcinoma (1/4,20%)decreased further(P<0.05).The expression of growth hormone rec eptor mRNA in cirrhotic liver tissue [ratio of integrated Optical Density(riOD)30.77%±8.24%,n=32] was significantly lower than that in normal control liver tissue(riOD 44.93%±6.25%,n=5)(P<0.05).The expression in severe cirrhotic liver tissue(riOD 21.90%±4.72%,n=8)decreased further(P<0.05).Conclusions:The down-regulated expression of growth hormone receptor mRNA was related to the pathological stage of hepatocellular cancer and the sta ge of liver cirrhosis.

关 键 词：肝肿瘤 肝硬化 生长激素受体 mRNA RT-PCR 肝细胞癌 癌旁肝硬化 

分 类 号：R735.7[医药卫生—肿瘤] R575.2[医药卫生—临床医学]