鼻咽癌细胞亚株不同成瘤与转移潜能的分子机制  

Molecular Mechanisms of Tumorgenesis and Metastasis in Nasopharyngeal Carcinoma Cell Sublines

作　　者：宋立兵[1] 鄢践[1] 简少文[1] 张玲[1] 李满枝[1] 李端[1] 汪慧民[1] 

机构地区：[1]中山大学肿瘤防治中心研究所,广东广州510060

出　　处：《癌症》2002年第2期158-162,共5页Chinese Journal of Cancer

基　　金：中山医科大学"211"工程项目(98040);广东省重点实验室项目(998077035)

摘　　要：背景与目的:我们已从鼻咽癌细胞株SUNE-1的克隆株中筛选出3个具有不同生物学特性的细胞亚株:5-8F(高成瘤、高转移)、6-10B(成瘤、不转移)和13-9B(不成瘤);在本研究中,我们从病毒和遗传两方面探讨鼻咽癌细胞株SUNE-1及其3个亚株的生物学特性差异的分子机制。方法:①PCR检测亚株中EB病毒(Epstein-Barrvirus,EBV)的BamHIW片段;②应用原位杂交技术检测SUNE-1及其3个亚株中EBV潜伏膜蛋白1(latentmembraneprotein1,LMP1)的表达状况;③应用比较基因组杂交(comparativegenomichybridization,CGH)检测SUNE-1及其3个亚株的基因扩增情况。结果:①在SUNE-1及其3个亚株中存在EBVBamHIW片段;②在3个亚株中,都能够检测到EBV-LMP1的表达,且表达强度一致。③CGH检测发现:SUNE-1的DNA拷贝数变呈现以1、2p、3、4、5p、6、7、9、10q、11、12q、13q、17q和18q增加和22q拷贝数减少为主;5-8F染色体变化以3p、7q、8q、9q、和10q拷贝数增加为主;而6-10B和13-9B除少数几个染色体区域发生缺失外,均无DNA拷贝数的增加。结论:鼻咽癌细胞株SUNE-1及其3个亚株,均有EBV的表达和存在不同的染色体变化,提示其生物学特性的差异可能主要由于染色体的变化引起,但EBV的感染对维持鼻咽癌的恶性程度可能起着重要的作用。Background &Objective:The authors have selected three diff erent biological character sublines,including 5-8F(the highest tumorigenic and metasta tic ability),6-10B(the lowest tumorigenicity and lack o f metastatic ability),and 13-9B(lack of tumorigenic ability),from colony lines of nasopharyngeal carcinoma cell line(SUNE-1).The aim of this study was to determine the molecular mechanisms of different b iological character of SUNE -1and it s three sublines from two aspects of genetic and virology.Method:1.Amplifying Epstein-Barr virus(EBV)BamHI Wfragment by PCR.2.Determining the expression o f latent membrane protein1(LMP1)of EBV in the sublines using in situ hybrization.3.Molecular cytogenetic analysis of t he cell line and sublines by comparative genomic hybridization(CGH).Results:1.There was EBV BamHI Wfragment in SUNE-1and its three sublines.2.Expression of LMP1was observed in the SUNE-1and its three sublines,and their expressio n intensity was identical.3.There were DNA copy number gains on chromoso mes 1,2p,3,4,5p,6,7,9,10q,11,12q,13q,and 18q as well as loss on 22q in the SUNE-1cell line;DNA copy number gains on chromosomes 3p,7q,8q,9q,and 10q we re observed in 5-8F subline;DNA copy number loss on few chromosomes,but no DNA copy number gain was observed in t he 6-10B and 13-9B sublines.Conclusion:There were expression of EBV and different genetic changes in the SUNE-1and its three sublines.It is sugg esting that the different biological character tumorgenesis and metastasis of naso pharyngeal carcinoma cell sublines may be due to genetic changes;and EBV infection may play an important role in keeping the malignant phenotypic of nasopharyngeal carcinoma.

关 键 词：鼻咽癌 细胞亚株 生物学特性 EB病毒 潜伏膜蛋白1 比较基因组杂交 转移潜能 分子机制 

分 类 号：R739.63[医药卫生—肿瘤]