阿尔茨海默病脑海马凋亡神经元发生率增高  

Increased apoptotic neurons in hippocampus of Alzheimer's disease

作　　者：许浩[1] 胡祥友[2] 秦松[2] 周江宁[1] 

机构地区：[1]中国科学技术大学生命科学学院神经生物学系,安徽合肥230026 [2]安徽医科大学第一附属医院安徽老年病研究所,安徽合肥230022

出　　处：《中国神经科学杂志》2002年第1期462-465,共4页

基　　金：国家重点基础研究发展规划项目资助 (G19990 5 40 0 7)

摘　　要：通过对阿尔茨海默病 (Alzheimer’sdisease ,AD)和老年对照患者死后脑海马凋亡神经元的检测 ,探讨AD脑神经元丢失的可能机制。使用TUNEL(terminaltransferase mediateddUTP biotinnickendlabeling)法对 7例确诊的AD和 9例老年对照患者死后海马组织中的凋亡神经元进行标记 ,用生物图像分析系统对结果进行定量分析。AD和对照组间TUNEL阳性细胞数无显著性差异 ;AD组海马CA1和CA4区正常神经元数减少 ,其中CA1区减少具有显著性差异 (P <0 .0 5 ) ;AD组海马CA1和CA4区凋亡神经元发生率高于对照组 ,其中CA1区具有显著性差异 (P <0 .0 5 )。AD组海马组织中凋亡神经元发生率明显增高 ,说明细胞凋亡可能参与了AD海马神经元退行性变的过程。The mechanism of degeneration of neurons in hippicampus of Alzheimer's disease (AD) patients was investigated. Apoptosis was detected by terminal transferase mediated dUTP biotin nick end labeling (TUNEL) in hippocampus of 7 AD patients and 9 age matched control, and quantitatively analyzed by biological image analysis system. No significant differences were found in total number of TUNEL positive cells between AD and control. Double labeling with TUNEL and neuron specific enloase (NSE) was used to identify the normal and apoptotic neurons, and it was found that the number of neurons in CA1 decreased significantly in AD compared with that in control ( P <0.05).The ratio of apoptotic neurons to total neurons was significantly higher in CA1of AD than that in control( P <0.05). higher ratio of apoptotic neurons in hippocampus of AD than in control. The results showed that apoptosis might play a role in neuron degeneration of AD. \[

关 键 词：阿尔茨海默病 细胞凋亡 海马 神经元 发生率 

分 类 号：R749.161[医药卫生—神经病学与精神病学]