重组人骨形成蛋白2对人牙周膜成纤维细胞的生物学作用  

The Biological Effects of Recombinant Human Bone Morphogenetic Protein 2 on Human Periodontal Ligament Fibroblasts

作　　者：司晓辉[1] 刘正[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学研究所,200011

出　　处：《华西口腔医学杂志》2002年第1期10-13,共4页West China Journal of Stomatology

基　　金：国家自然科学基金资助项目 (编号 3 0 0 0 0 191)

摘　　要：目的 :研究重组人骨形成蛋白 2 (rhBMP2 )对人牙周膜成纤维细胞 (HPDLFs)的生物学作用。方法 :原代培养HPDLFs,并观察rhBMP2对HPDLFs增殖、碱性磷酸酶 (ALP)活性、骨钙素 (OC)合成及矿化结节形成的作用。结果 :0 2 5～ 2mg mlrhBMP2对HPDLFs的增殖无明显作用 ,0 5～ 2mg mlrhBMP2显著提高HPDLFs的ALP活性 ,刺激OC合成和矿化结节形成。结论 :rhBMP2对HPDLFs的作用具有剂量依赖性。Objective:The purpose of this study is to study the biological effects of recombinant human bone morphogenetic protein 2 (rhBMP2) on human periodontal ligament fibroblasts (HPDLFs).Methods: Human periodontal ligament fibroblasts were primary cultured and detected the different doses of rhBMP2 on their proliferation, alkaline phosphatase (ALP) activity, osteocalcin (OC) synthesis and formation of the mineralized nodules.Results: rhBMP2 (0 25～2 mg/ml) had no remarkable effect on the proliferation of HPDLFs. The ALP activity, OC synthesis and formation of the mineralized nodules of HPDLFs were significantly stimulated by 0 5～2 mg/ml rhBMP2.Conclusion: The effects of rhBMP2 on HPDLFs are dose_dependent. Not only can rhBMP2 stimulate the expression but also promote the maturation of osteoblastic phenotype of HPDLFs.

关 键 词：重组人骨形成蛋白2 人牙周膜成纤维细胞 碱性磷酸酶 骨钙素 矿化 牙科材料 生物学作用 RHBMP2 

分 类 号：R780.2[医药卫生—口腔医学]