人肿瘤坏死因子-α基因转染与干扰素-γ联合应用对舌癌细胞生长的影响  

Synergistic Effects of Human Tumor Necrosis Factor-α Gene Transfection and Interferon-γ on the Growth of Tongue Carcinoma Cells

作　　者：高振南[1] 李声伟[1] 高家让[1] 田卫东[1] 刘磊[1] 

机构地区：[1]四川大学华西口腔医学院口腔颌面外科学教研室,610041

出　　处：《华西口腔医学杂志》2002年第1期55-57,共3页West China Journal of Stomatology

基　　金：卫生厅科学研究基金资助项目 (编号 960 0 3 9)

摘　　要：目的 :探讨干扰素_γ(IFN_γ)与人肿瘤坏死因子_α(hTNF_α)基因转染联合应用对舌癌细胞生长的影响。方法 :将培养细胞分为 2组 ,其中一组转染hTNF_α基因 ,另一组不转染 ;再将每一组又分为 5个小组 ,每一小组分别加入IFN_γ ,使其终浓度分别为 0、1、10、10 0、10 0 0U/ml,培养 4 8h后 ,用MTT法测定Tca8113细胞的存活率 ,用免疫细胞化学染色法观察hTNF_α的表达。结果 :单纯加入IFN_γ对舌癌细胞的活性无影响 ;hTNF_α基因转染与IFN_γ联合应用时 ,不同浓度的IFN_γ均有协同hTNF_α基因转染杀伤舌癌细胞的作用 ,而且其抑瘤效应与IFN_γ的浓度呈正相关(r=0 86 7,P <0 0 1) ;与未转染组相比 ,转染组细胞hTNF_α呈明显的高表达。结论Objective: The purpose of this study is to investigate the synergistic effects of human tumor necrosis factor_α (hTNF_α) transfection and interferon_γ (IFN_γ) on the growth of tongue carcinoma cells.Methods: The cultured Tca8113 tongue carcinoma cells was divided into 2 groups, one group was transferred with hTNF_α gene. Each of the 2 groups was then divided into 5 subgroups, and the subgroups were added IFN_γ until the final IFN_γ concentrations respectively were 0, 10, 100, and 1000 U/ml. After culturing for 48 hours, the survival rates of the all groups of cells were assayed by MTT enzymatic labeling technique, and the expression of hTNF_α in Tca8113 cells was observed with immunocytochemistry.Results: IFN_γ did not affect the growth of Tca8113 cells without hTNF_α, however, the transfection of hTNF_α with the above different concentrations of IFN_γ synergistically inhibited the growth of Tca8113 cells, the concentrations of IFN_γ were positively correlated with the inhibition effects (r=0.733,P<0.01), the transferred Tca8113 cells displayed remarkable overexpression of hTNF_α, compared with the non_transferred.Conclusion: IFN_γ can enhance the inhibition effects of hTNF_α transfection on the tongue carcinoma cells.

关 键 词：人肿瘤坏死因子-Α 干扰素-Γ 基因转染 舌癌 细胞生长 影响 

分 类 号：R739.86[医药卫生—肿瘤]