腱蛋白在四氯化碳大鼠肝纤维化形成过程中的动态表达  

Dynamic expression of tenascin in rat liver during liver fibrogenesis induced by CCl_4

作　　者：姚定康[1] 李石[1] 孔宪涛[2] 叶挺军[3] 

机构地区：[1]上海第二军医大学附属长征医院消化科,200003 [2]上海第二军医大学附属长征医院临床免疫中心,200003 [3]上海第二军医大学附属长征医院病理科,200003

出　　处：《中华肝脏病杂志》2002年第1期40-42,共3页Chinese Journal of Hepatology

摘　　要：目的 探讨腱蛋白（Ｔｅｎａｓｃｉｎ，Ｔｎ）在肝纤维化形成中的作用。方法 以ＣＣｌ４诱发大鼠肝损伤期（４周）、肝纤维化早期（８周）和肝纤维化晚期 （１２ 周）模型；采用免疫组织化学及核酸原位分子杂交技术对大鼠肝组织中Ｔｎ ｍＲＮＡ及其蛋白质表达进行观察，并进行计算机图像分析。结果 正常肝组织有少量Ｔｎ分布；肝损伤期及肝纤维化早期，Ｔｎ的ｍＲＮＡ及蛋白质表达均显著增高。肝纤维化晚期，Ｔｎ的ｍＲＮＡ及蛋白质表达相对减少。核酸原位杂交提示肝间质细胞是Ｔｎ的主要合成细胞。结论Ｔｎ是肝内重要的细胞外基质成分，参与了肝纤维化的早期形成。Objective To study the expression of tenascin in normal and fibrotic rat liver. Methods Liver fibrosis induced in rat with CCl4 were divided into three stages: the stage of hepatic injury (4 weeks), early stage of hepatic fibrosis (8 weeks) and later stage of hepatic fibrosis (12 weeks). Tenascin expression in liver tissue was observed by immunohistochemical method and in situ hybridization using digoxigenin-labelled DNA probe. Results In normal rat liver there was a weak staining for tenascin. In both liver injury stage and early stage of hepatic fibrosis, both mRNA signal and immunostaining for tenascin were significantly increased as compared to that in normal liver. In later stage of hepatic fibrosis. mRNA signal and immunostaining for tenascin were decreased compared with that in early stage of hepatic fibrosis. The cellular source of tenascin in liver mainly restricted in mesenchymal cells. Conclusions Tenascin is a component of the extracellular matrix of liver tissue. Plays a role in early matrix organization during liver fibrogenesis.

关 键 词：肝纤维化 免疫组织化学 原侠杂交 腱蛋白 四氯化碳 大鼠 

分 类 号：R575.2[医药卫生—消化系统]