海嘧啶对SGC-7901人胃癌细胞凋亡的影响  

Influence of sea pyrimidine on apoptosis of SGC-7901 human stomach cancer cells

作　　者：季宇彬[1] 高世勇[1] 孔琪[1] 张秀娟[1] 杨宝峰[2] 

机构地区：[1]哈尔滨商业大学制药工程系,黑龙江哈尔滨150076 [2]哈尔滨医科大学,黑龙江哈尔滨150086

出　　处：《哈尔滨工业大学学报》2002年第1期91-94,124,共5页Journal of Harbin Institute of Technology

基　　金：国家科委生命中心博士基金资助项目 (96 90 1 0 6 10 );中国优秀博士后基金资助项目 (中博号 [1999] 17);黑龙江省杰出青年基金资助项目 (J990 6);黑龙江省自然科学基金资助项目 (D980 1) .

摘　　要：为了揭示海嘧啶的抗肿瘤作用机制 ,采用流式细胞仪测定海嘧啶对人胃癌细胞凋亡及细胞周期的影响 ,采用激光扫描共聚焦技术观测海嘧啶对人胃癌细胞内 [Ca2 + ]i的影响及 [Ca2 + ]i变化时Ca2 + 的来源 .海嘧啶可诱导肿瘤细胞凋亡 ,海嘧啶可升高肿瘤细胞内 [Ca2 + ]i的浓度 ,Ca2 + ]i升高时Ca2 + 来源于细胞外钙内流和细胞内钙释放 .海嘧啶的抗肿瘤机制为诱导肿瘤细胞凋亡 ,海嘧啶通过开放细胞膜钙通道和引起细胞内钙释放两条途径升高肿瘤细胞内 [Ca2 + ]i,启动细胞凋亡机制 ,从而诱导肿瘤细胞凋亡 .The influence of sea pyrimidine on the apoptosis and life cycle of human stomach cancer cells, and the variation of [Ca 2+ ] i levels as well were observed by FCM using the laser scanning confocal microscope. Sea pyrimidine was found to be able to induce the apoptosis of tumor and increase the [Ca 2+ ] i level in tumor cells by opening the pathway to release calcium. It is concluded that the anti tumor mechanism of sea pyrimidine is that the apoptosis of tumor cells is induced by the increased [Ca 2+ ] i level in tumor cells resulting from the releasing of calcium through the opened pathway.

关 键 词：海嘧啶 细胞凋亡 胃癌 癌细胞 抗肿瘤 

分 类 号：R735.2[医药卫生—肿瘤] R979.1[医药卫生—临床医学]