苯那普利对阿霉素肾病幼鼠肾硬化的保护作用  

The protective effects of Benazepril on glomerulosclerosis in young rats with adriamycin induced nephropathy

作　　者：胡波[1] 李红叶[2] 杨霁云[3] 丁洁[3] 张敬京[3] 

机构地区：[1]安徽医科大学第一附属医院儿科 [2]河南医科大学第一附属医院儿科 [3]北京大学第一医院儿科,北京100034

出　　处：《临床儿科杂志》2002年第2期98-100,T001,共4页Journal of Clinical Pediatrics

基　　金：中华医学基金会医学师资奖学金 (CMB)资助项目 (编号 :93593)

摘　　要：为探讨苯那普利对阿霉素肾病幼鼠肾硬化的保护作用 ,将SD幼龄大鼠行单侧肾切除加阿霉素注射建立动物模型 ,术后即对治疗组大鼠给予苯那普利6mg/(kg·d)治疗12周 ,观察大鼠尿蛋白、血生化及残余肾组织的病理改变。结果 :苯那普利于治疗的第7、9、12周显示出较好降尿蛋白作用 ,尿蛋白与同期模型组相比分别为 (7.3±4.4)mg/24h、(2.0±1.2)mg/24h、(5.2±3.5)mg/24h比 (14.4±1.8)mg/24h、(17.5±9.8)mg/24h、(15.2±4.7)mg/24h(P均<0.01) ;形态学上显示系膜增生和肾小球硬化均减轻 ,与模型组相比系膜增生率和硬化指数分别为8.0(5.0～13.1) %比35.4(11.0～67.5) %和0.3(0.3～0.9)比1.9(0.3～6.7) (P均<0.05)。提示 ,在单侧肾切除加阿霉素注射的幼龄肾病大鼠肾硬化模型中 ,苯那普利显示了肾保护作用。AngiotensinⅡis thought to be a crucial factor in inducing the progressive renal injury.Benazepril,a new type of angiotensin converting enzyme inhibitor (ACEI),can inhibit the angiotensin Ⅱ activity fairly well through inhibiting angiotensin converting enzyme in circulation and tissues.Thus,it has the protective effect on glomerulosclerosis.Most of these findings came from the studies in adult animal and human beings.However,up to now,the renal protective effect of ACEI has not been extensive studied in fawn_hood and children at home.To explore the protective effects of Benazepril on glomerulosclerosis in the young rat with adriamycin induced nephropathy,all experiments were performed on male SD rats (one month age ,weight 100g±).Sixteen rats were divided into three groups:control group(n=5),model group(n=6)and Benazepril_treated group(n=5).Sham operation was performed and one week after ,the normal saline was injected through the tail vein.The animal model with glomerulosclerosis was established with uninephrectomy and adriamycin (5mg/kg)injection in the other 2 groups.After uninephrectomy,Benazepril 6mg/(kg·day) was given in the Benazipril_treated group for 12 weeks.Proteinuria and some biochemical values in serum were assayed at the regular intervals and the pathological changes in the remnant kidney were investigated at the end of the experiments.The results indicated the effect on reducing the proteinuria at 7,9, and 12 weeks in the Benazepril group wes greater than that in the model group (proteinuria 7.4±4.4mg/24h VS 14.4±1.8mg/24h,2.0±1.2mg/24h VS 17.5±10.0mg/24h and 5.2±3.5mg/24h VS 15.2±4.7mg/24h respectively,P<0.01),only a mild mesangial proliferatlon [mesangal expansion rate 8.0%(5.0%～13.1%) VS 35.4%(11.0%～67.5%),P>0.05] and glomerulosclerosis [sclerosis index 0.3(0.3～0.9) VS 1.9(0.3～6.7),P<0.05] could be found in the Benazepril_treated group(P<0.05) suggesting that Benazepril have the renal protecfive effects on proteinuria and glomerular sclerosis in the young rat model with adri

关 键 词：苯那普利 幼鼠 肾硬化 保护作用 阿霉素 

分 类 号：R726.9[医药卫生—儿科]