HLA-II类抗原基因分型PCR-SSP方法在ALLo-HSCT中的应用  

The Application of HLA Class II Genotyping by Polymerase Chain Reaction Amplification with Sequence-specific Primers Method in Allogeneic Hematopoietic Stem Cell Trasplantation

作　　者：魏彩霞[1] 杨绵本[1] 李玛琳[2] 景强[2] 黎承萍[1] 杨弘[1] 肖敏[1] 

机构地区：[1]昆明医学院第一附属医院血液科,昆明650032 [2]昆明医学院,昆明650031

出　　处：《昆明医学院学报》2002年第1期48-51,共4页Journal of Kunming Medical College

基　　金：云南省教育厅科研基金资助项目 (9812 14 )

摘　　要：准确的HLA配型是异基因造血干细胞移植 (Allo -HSCT)成功的关键之一 ,经典的血清学方法用于HLA -II类抗原的配型因存在诸多不足可导致漏配或错配 ,为了克服血清学方法的种种不足 ,我们建立了HLA -II类聚合酶链反应 -序列特异性引物 (PCR -SSP)基因配型方法 .此方法可避开血清学方法的种种干扰 ,直接从基因水平检测HLA的个体遗传学差异 .结果表明 :PCR -SSP方法具有重复性好、特异性强、灵敏度高、实验结果易于判断等特点 ,对于Allo -HSCT选择理想供者 ,减少或减轻Allo -HSCT时GVHD的发生 ,有着至关重要的意义 .可满足Allo -HSCT对HLA配型准确性高的要求 ,为在同胞兄弟姐妹间和无血缘关系供Precise matching of human leucocyte antigen (HLA) is the key step to successful Allo-HSCT. Classical serologic method applied in HLA class II tissue typing may result in mismatch because of some defects. The method of HLA-II genotyping by polymerase chain reaction-sequence specific primers (PCR-SSP) was established in order to overcome shortcomings of serology. It can avoid a lot of serologic interference. Individual difference in genetics of HLA can be examined directly on genic level. Results show that PCR-SSP method is charactered with high reproducibility, specificity, sensitivity and easy analysis to experimental results. These features are very important for selecting ideal donors of Allo-HSCT and they can decrease the incidence of GVHD. The method can reach the goal of accurate HLA genotyping in Allo-HSCT. It can be guaranteed that ideal donors will be selected among sibling and unrelated donor-recipient.

关 键 词：异基因造血干细胞移植 聚合酶链反应-序列特异性引物 HLA-Ⅱ类抗原 基因分型 血清学 PCR-SSP 恶性血液病 ALL0-HSCT 

分 类 号：R733[医药卫生—肿瘤]