免疫异常肝坏死动物模型的建立  

Establish of Massive Liver Cell Necrosis Model by P.acnes and LPS in Mice

作　　者：刘沛[1] 吴海滨[1] 王兆荃[1] 宋军[1] 

机构地区：[1]中国医科大学第二医院传染科,锦州市传染病院

出　　处：《胃肠病学和肝病学杂志》1994年第4期280-283,共4页Chinese Journal of Gastroenterology and Hepatology

基　　金：国家自然科学基金

摘　　要：本研究利用丙酸杆菌和内毒素建立了肝坏死动物模型。其结果：①２４小时死亡率为８３％（２０／２４）。肝组织学检查所见肝小叶中有大量单核吞噬细胞浸润，并出现大片肝坏死；②脾细胞对ＰＨＡ和ＣｏｎＡ反应明显降低；③肝枯否氏细胞肿瘤坏死因子和白细胞介素１生成能明显增高；④白细胞介素２不能调节脾细胞对ＰＨＡ的反应。上述结果说明此模型与免疫异常有着密切关系，是研究肝坏死免疫发生机理的较理想模型。In present studies, we established the model of massive liver cell necrosis in mice which were injected with propionibacterium acnes (P. acnes) and subsequently 7 days later with a small dose of lipopolysaccharide-endotoxin (LPS). The findings were as follows:①The mortality was 83% after 24 h of the injection of LPS in the model. The infiltration of mononuclear cells and massive liver cell necrosis were found in the liver lobules. ②The proliferative spleen cells of P. acnes treated-mice responding to PHA or ConA were significantly lower than that of control mice,③The productions of tumor necrosis factor and interleukinlin the supernates of culture of Kupffer cell dissociated P. acnes treated-mice were significantly higher than that of control mice.④Influences of interleukin-2 enhancing proliferative spleen cells dissociated P. acnes treated-mice responding to PHA were not found. These results strongly suggest that the abnormal immunity involved in the liver cell necrosis in the model.

关 键 词：肝坏死 内毒素 免疫异常 动物模型 

分 类 号：R575.230.2[医药卫生—消化系统] R－332[医药卫生—内科学]