老年2型糖尿病APOE基因多态与血浆连结蛋白和蛋白C关系探讨  

To explore on relationship between APOE genotype and Protein C Fibronection in type 2 diabetic disease

作　　者：沈庆乐[1] 陈兴雅[1] 李培英[1] 林闽溪[1] 林美明[1] 吴青平[1] 庄梁[1] 

机构地区：[1]三明市第一医院,365000

出　　处：《福建医药杂志》2002年第1期75-77,共3页Fujian Medical Journal

摘　　要：目的　研究 APOE基因多态与血浆连结蛋白 C(PC)的关系。方法　应用聚合酶链反应 -限制性片段多态性 (PCR- RFCP)技术与应用 EL ISA法测定 PC、 Fn35例老年糖尿病 2型患者。对照组为正常健康人 5 0例。结果　糖尿病组以 3/ 4型基因为主 ,健康对照组以 3/ 3型基因分布频率最高。 DM2型、 DM2型并心脑血管病组的等位基因频率分别为 0 .18、 0 .0 3、 0 .79;0 .0 6、 0 .0 6 ,0 .88。糖尿病组基因分布 E3/ 4 >E2 / 3>E3/ 3。PC、Fn与 ε4 呈正相关。结论　APOE4 等位基因携带可能是糖尿病的重要遗传标志。在糖尿病发生。objective To study the relationship ApoE genotype and PC.Fn in type 2 diabetic disease. Methods ARMS technique was used.Polymerase chain reaction and restrictive fragment length polymorphism[PCR RFLP] to determine DNA APOE genotype in 35 type 2 diabetic disease. An 55 control subjects without and sign of Type 2 diabetic disease. Protein C and Fn measured by ELISA .Results Conmared with the healthy subject ε3/4 genotype and ε frequency were increased in DM group.The frequencies of E 2?E 3?E 4 alleles were separately 0 18, 0 03.,0 79 in control group and 0 06, 0 06, 0 88 in the DM group.Conclusion The data indicate apolipoprotein E?ε 4 allele is biological risk factor for DM. and ε3/4 genotype may be associated with DM.

关 键 词：Ⅱ型糖尿病 APOE基因 基因频率 等位基因 聚合酶链反应 老年人 基因多态性 蛋白C 血浆连结蛋白 

分 类 号：R587.1[医药卫生—内分泌]