碳源和Mn^(2+)对地衣芽孢杆菌Bacillus lic heni for misWBL-3生产聚γ-谷氨酸的影响  

EFFECTS OF CARBON SOURCE AND MANGANESE ION ON PRODUCTION OF γ-POLY(GLUTAMATE) BY Bacillus licheniformis WBL-3

作　　者：杨革[1] 陈坚[1] 曲音波[2] 伦世仪[1] 

机构地区：[1]无锡轻工大学生物工程学院环境生物技术室,江苏无锡214036 [2]山东大学微生物技术国家重点实验室,山东济南250100

出　　处：《化工学报》2002年第3期317-320,共4页CIESC Journal

基　　金：教育部高等学校重点实验室访问学者基金资助项目(No .Y660 0 2 )

摘　　要：The effects of carbon source and manganese ion on the production of γ -poly (glutamate) ( γ -PGA) was investigated.The results showed that the production of γ -PGA was different on varions carbon sources, and among those, citric acid and glycerol were much better than others.The production of γ -PGA was increased to 15.72?g·L -1 by using the citric acid and glycerol as a combined carbon source.It was also found that the production of γ -PGA could be regulated by control of manganese ion concentration in the medium.Without Mn 2+ ,there was no γ -PGA produced.The production of γ -PGA was increased by increasing Mn 2+ concentration, the optimal concentration of Mn 2+ was 39.0?mg·L -1 .The composition and morphology of γ -PGA were also affected by Mn 2+ .The effects of carbon source and manganese ion on the production of γ -poly (glutamate) ( γ -PGA) was investigated.The results showed that the production of γ -PGA was different on varions carbon sources, and among those, citric acid and glycerol were much better than others.The production of γ -PGA was increased to 15.72?g·L -1 by using the citric acid and glycerol as a combined carbon source.It was also found that the production of γ -PGA could be regulated by control of manganese ion concentration in the medium.Without Mn 2+ ,there was no γ -PGA produced.The production of γ -PGA was increased by increasing Mn 2+ concentration, the optimal concentration of Mn 2+ was 39.0?mg·L -1 .The composition and morphology of γ -PGA were also affected by Mn 2+ .

关 键 词：碳源 地衣芽孢杆菌 多聚γ-谷氨酸 锰(Ⅱ) 发酵法生产 BacilluslicheniformisWBL-3 培养条件 

分 类 号：TQ922.1[轻工技术与工程—发酵工程]