蛋白质结构型的定义和识别  

The Definition and Recognition of Protein Structural Class

作　　者：李晓琴[1] 罗辽复[1] 

机构地区：[1]内蒙古大学理工学院,呼和浩特010021

出　　处：《生物化学与生物物理进展》2002年第1期124-127,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金资助项目 (39960 0 23)~~

摘　　要：提出紧结构域的概念 ,由二级结构序列中一段或几段连续的α螺旋和 β折叠构成的空间紧密堆集的最大折叠体称为紧结构域 .利用 3种紧结构域 (α域 ,β域和α/β域 )定义球蛋白的 5种结构型 :α型蛋白 ,β型蛋白 ,α/β型蛋白 ,多域蛋白和 ζ型蛋白 .将 12 6 1个代表性的蛋白质 (10 2 2家族 )进行分类 ,并和SCOP库的分类做了比较 .进行了删去序列冗余的分析 .在此基础上提出结构型的预测方案 ,成功率在 82 %～ 85 %.The concept of compact structural domain (CSD) is proposed which means a maximal compactly - packing - fold composed of alpha helices and beta sheets in secondary structure sequence. There are three kinds of CSDs, namely alpha domain, beta domain and alpha/beta domain. Then five classes of protein are defined. The mainly-alpha protein is constructed from one or several a domains, the mainly-beta protein is constructed from one or several beta domains, the alpha/beta protein is constructed from one or several alpha/beta domains, the multi-domain protein is constructed from two or more kinds of CSDs, and the zeta-class protein does not contain any CSD. A database of 1 261 globular proteins is classified into five classes. The classification is compared with SCOP's. The analysis of redundancy-deletion has been done. A prediction rule on structural class is given which is the generalization of previous work. The successful rate of the prediction is higher than 82%.

关 键 词：蛋白质结构型 二级结构序列 紧结构域 序列冗余 预测 识别 

分 类 号：Q51[生物学—生物化学]