注射大肠杆菌对柞蚕滞育蛹血浆中游离氨基酸的影响  

Changes of the Free Amino Acids in the Plasma of Antheraea pemyi Diapausing Pupae in Relation to Injection of Escherichia coli

作　　者：欧阳迎春[1] 王宗舜[1] 关雪辰[1] 

机构地区：[1]中国科学院动物研究所农业虫鼠害综合治理研究国家重点实验室,北京100080

出　　处：《农业生物技术学报》2002年第1期20-23,共4页Journal of Agricultural Biotechnology

摘　　要：本实验检查了柞蚕（Antheraea pernyi）滞育蛹和滞育后蛹血浆内游离氨基酸滴度,以及滞育蛹注射大肠杆菌E.coli后所引起的血浆游离氨基酸的变化。在滞育蛹血浆内,脯氨酸滴度最高,分别占总游离氨基酸的25.8％（雌）和19％（雄）,这可能与越冬柞蚕耐寒性有关;苏氨酸和丙氨酸在滞育蛹血浆内的含量也相当丰富。表明脯氨酸,苏氨酸和丙氨酸的高滴度是柞蚕滞育蛹的特征。注射大肠杆菌1h后,滞育蛹血浆内总游离氨基酸浓度增加10％以上,而氨基酸组成类型没有明显改变,说明游离氨基酸浓度的增加是对入侵细菌的一种免疫反应,并为抗菌物质的合成作准备。Three titers of free amino acids present in the plasma of diapausing and post-diapausing pupae of oak silkworm, Antheraea pemyi,and their changes in the diapausing pupae induced by injection of E.coli were examined.The liter of proline in the plasma of diapausing pupae were the highest and 25.8%(female)and 19% (male)in the total free amino acids respectively, which might contribute to the cold tolerance of this species. Threonine and alanine conlenl in the diapausing pupae plasma was quite abundanl.The high liters of proline,threonine and alanine appear to be characteristic of the diapausing pupae of A.pernyi. 1 h after injection of E. coli ,the tolal free amino acids in the plasma of diapausing pupae increased over 10%, but there was little change in free amino acid composition pattern as compared with untreated ones.It suggesls that increase of the total free amino acid concentration in the plasma is an immunological response to the invaded bacteria and may be ready to synthesize some inducible antibacterial substances.

关 键 词：游离氨基酸 血浆 滞育蛹 柞蚕 大肠杆菌 注射 抗菌物质 免疫因子 昆虫 

分 类 号：Q966[生物学—昆虫学] Q965.8