对虾WSSV人工感染螯虾及其检测  

ARTIFICIAL INFECTION OF CRAYFISH WITH WSSV OF PENAEID SHRIMP AND ITS DETECTION

作　　者：莫照兰[1] 雷质文[2] 杨冰[2] 黄倢 张培军[1] 

机构地区：[1]中国科学院海洋研究所实验海洋生物学开放实验室,青岛266071 [2]中国水产科学研究院黄海水产研究所农业部增养殖病害与生态重点开放实验室,青岛266071

出　　处：《海洋科学》2002年第3期43-46,共4页Marine Sciences

基　　金：国家"九五"重点科技攻关资助项目96 005 03 01 03号

摘　　要：用对虾白斑综合症病毒(WSSV)人工感染淡水克氏原螯虾(Procambarusclarkii) ,感染组螯虾3～6d内全部死亡。核酸探针点杂交两次检测感染螯虾的鳃、胃、血淋巴 ,阳性检出率分别为92% (92% ) ,89 % (86 % ) ,81 % (75 % ) ,对照为11 % (3% ) ,5 % (5 % ) ,0(0) ;光镜下可观察到感染螯虾的胃、鳃组织的靶细胞核肿大、嗜酸性着染 ,电镜下病变组织细胞核可见形态大小与WSSV一致的病毒粒子 ;病毒核酸原位杂交两次检测感染螯虾的胃、鳃 ,阳性检出率均为100 % ,对照阳性检出率均为0。结果表明 :对虾WSSV可感染淡水克氏原螯虾 。Healthy freshwater crayfish (Procambarus clarkii) were infected with the virus of white spot syndrome virus (WSSV) of penaeid shrimp, and the diseased crayfish died within 3 6 days. Histopathological studies of light and electronic microscopy examination indicated that the tissues of gill and stomach in diseased crayfish were infected by baculovirus, whereas DNA dot blot hybridization and in situ hybridization assay confirmed that the gill, stomach and hemolymph of diseased crayfish were positively infected by WSSV. So the crayfish were certainly infected by WSSV, and it is demonstrated that in situ hybridization assay was specific and sensitive to detect WSSV.

关 键 词：WSSV 人工感染 克氏原螯虾 核酸探针点杂交 核酸原位杂交 检测 对虾病毒 

分 类 号：S945.19[农业科学—水产养殖]