RhoA基因在结直肠癌组织中的表达  

RhoA gene expression in colorectal carcinoma

作　　者：王颢[1] 陈玉霞[1] 曹冬梅[1] 张晔[1] 卢建[1] 孟荣贵[2] 

机构地区：[1]第二军医大学病理生理教研室,上海200433 [2]长海医院肛肠外科

出　　处：《中华医学杂志》2002年第5期348-351,共4页National Medical Journal of China

摘　　要：目的　研究RhoA基因mRNA在结直肠癌中的表达。方法　建立测定RhoA基因mRNA表达的逆转录聚合酶链反应 (RT PCR)最适条件 ,在半定量水平测定 42例结、直肠癌手术标本正常粘膜组织、癌组织的RhoA基因mRNA表达的相对水平 ,探讨与临床病理学指标的关系。结果　 42例结、直肠癌中RhoA基因表达较相应正常粘膜组织高 ,差异有非常显著意义 (P <0 .0 1 )。以 42例肿瘤与正常粘膜表达量比值平均值为界 ,将患者分为过度表达组和高表达组 ,过度表达组中淋巴结转移发生率较高 ,肿瘤分期较晚 (P <0 .0 5)。结论　RhoA基因在结、直肠癌进展中起重要作用 。Objective To investigate the expression of RhoA mRNA in colorectal neoplasms. Methods Total RNA was extracted from 42 surgically resected specimens of colorectal caricinoma and normal mucosa close to the lesions. Afier the optimal reverse transcription polymerase chain reaction (RT PCR) condition was found, the mRNA expression levels of the RhoA gene in the lesions and the normal mucosa were examined semi quantitatively and the relation between these expression levels and clinical pathological markers was compared. Results The expression of RhoA gene mRNA in lesion of colorectal carcinoma was significantly higher than that in contiguous normal mucosa (1.8±0.6 vs 0.9±0.4, P <0.01). The ratio of RhoA expression in neoplasm to that in contiguous mucosa was 2.02±0.82. Based on this ratio the 42 patients were divided into two groups: overexpression group (19 cases) and high expression group (23 cases). In the overexpression group the lesions of 14 cases were in Dukes C and Dukes D stages, and lymph node metastasis was found in 13 cases. In the high expression group, only 9 out of the 23 cases were in advanced stage and 8 cases showed lymph node metastasis. Chi squared test analysis demonstrated a significant difference in both aspects between these two groups ( P <0 05). The difference in number of metastatic lymph nodes between the two groups was statistically significant ( P <0.01). Mutation was not found in the RhoA gene sequence examined. Conclusion RhoA gene may play an important role in colorectal carcinogenesis and correlate with invasion and metastasis of colorectal carcinoma.

关 键 词：基因表达 结直肠肿瘤 肿瘤转移 结肠肿瘤 结肠癌 直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]