小鼠肝癌总RNA转染的树突状细胞体外诱导特异性细胞毒T淋巴细胞的研究  

Anti-tumor responsiveness by dendritic cells transfected with tumor total RNA

作　　者：王国强[1] 钟翠平[1] 傅继东[1] 范强[1] 张新华[1] 吴超群[1] 顾云娣[1] 

机构地区：[1]复旦大学医学院组织学与胚胎学教研室,上海200032

出　　处：《中国免疫学杂志》2002年第2期102-105,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金 (3 9770 3 87);1999年高校博士点基金(983 9)资助

摘　　要：目的 :探讨小鼠骨髓树突状细胞 (dendriticcell,DC)体外经Hepa 1 6肝癌细胞株总RNA转染后 ,对特异性细胞毒T淋巴细胞 (CTL)的诱导作用。方法 :自小鼠骨髓分离DC前体细胞 ,经GM CSF +IL 4培养、扩增 ;制备Hepa 1 6小鼠肝癌细胞株总RNA ,体外转染DC ,检测DC诱导同基因型小鼠T细胞增殖及其特异性CTL的反应能力。结果 :经Hepa 1 6肝癌细胞总RNA转染的DC ,其组织相容性分子 (MHC I、II)及共刺激分子 (B7 1 、B7 2 )表达明显增高 ,刺激同基因型小鼠T细胞增殖能力增强 ,且能诱导Hepa 1 6特异性CTL。结论 :以肝癌总RNA转染DC ,构造肝癌疫苗为肝癌的临床治疗提供了新的策略。Objective:The present study was designed to investigate whether transfecting DC with tumor derived total RNA is an effective way to induce CTL and antitumor immunity.Methods:DC were propagated from bone marrow(BM) of C57BL/6J(H 2k b?I A b)mice in vitro with GM CSF+IL 4.Tumor derived total RNA extracted from actively growing Hepa 1 6 cells was used to transfected DC.The phenotypes of DC were detected by FACS,the cytotoxicity of CTL was assayed by MTT method.Results:The tumor derived total RNA transfected DC exhibited much more and longer plasm membrane processes and increased expression of MHC Ⅰ?MHC Ⅱ?CD80(B7 1)?CD86(B7 2).Conclusion:This experiment has shown that DC transfected with tumor derived total RNA of C57BL/6J cells could stimulate effectively the responsiveness of syngenic splenic T cells to induce specific CTL against C57BL/6J cells.

关 键 词：树突状细胞 肝癌 核糖核酸 细胞毒T淋巴细胞 动物实验 RNA转染 

分 类 号：R735.7[医药卫生—肿瘤] R73－362[医药卫生—临床医学]