肿瘤源性早孕因子的筛检、纯化及鉴定  

Screening,purification and identification of tumor-derived early pregnancy factor

作　　者：张冬云[1] 王家骥[2] 杨英[3] 林丽白[2] 冯苏妹[2] 苏宝田[4] 

机构地区：[1]广州医学院生物化学教研室,广东广州510182 [2]广州医学院预防医学教研室,广东广州510182 [3]广州医学院微生物教研室,广东广州510182 [4]安徽医科大学,安徽合肥230022

出　　处：《中国生化药物杂志》2002年第1期3-6,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：广东省自然科学基金 ( 970 112 )

摘　　要：目的筛检出具有t EPF样活性的肿瘤样本 ,并对其进行分离纯化。方法用活性玫瑰花结抑制实验进行筛检 ,采用DEAE纤维素离子交换色谱、S SepharoseF .F离子交换色谱、ConA SepharoseCl 4B亲和色谱、Heparin SepharoseCl 6B亲和色谱等方法进行纯化 ,用SDS PAGE测定其相对分子量 ,以等电聚焦电泳法检测其等电点。结果膀胱癌、黑色素瘤、绒癌、恶性葡萄胎 4种肿瘤血清和恶性葡萄胎清宫人流血及黑色素瘤标本组织液具有t EPF活性。蛋白含量为 0 .49mg/ml,相对分子量为 134 2 4、2 32 19、382 73,等电点为 6 .5 3。结论 4种肿瘤血清和 2种肿瘤组织液中纯化获得的t EPF在相对分子量、等电点等生化指标上完全一致 。PurposeThe aim is to screen tumor samples with tumor derived early pregnancy factor (t EPF) activity, and to extract and to purify t EPF from the samples. MethodsThe samples that contain t EPF activity were screened by using Ea rosette inhibition test. The t EPF was purified by DEAE ion exchange cellulose, S sepharose F. F ion exchange , ConA sepharose Cl 4B affinity and Heparin sepharose Cl 6B affinity chromatographies. The peptide diagrams were examined by SDS PAGE. The pI of t EPF was measured by IEF. ResultsThe t EPF activity was found in the serum of four tumors bladder cancers,malignant melanoma,choriocarcinoma,malignant hydatidiform mole , as well as in the blood of the patients with malignant hydatidiform mole collected during artificial abortion and tissue fluid of malignant melanoma sample. The relative molecular weights of t EPF are 13 424, 23 219 and 38 273 Dalton.ConclusionSix t EPFs purified from two kinds of tumor and four kinds of tumor serum respectively had the same pI and relative molecular weight, and there was not any fundamental difference between tumor derived EPF and fetal derived EPF.

关 键 词：肿瘤源性早孕因子 纯化 筛检 鉴定 肿瘤生物标志物 SDS-PAGE测定 

分 类 号：R730.4[医药卫生—肿瘤]