日本血吸虫新基因Sj Dad1的DNA免疫动物保护性实验研究  

Evaluation of protection of BALB/c mouse against Schistosoma japonicum infection through DNA immunization of Sj Dad1

作　　者：李焱[1] 余新炳[1] 吴忠道[1] 孟玮[1] 陈慧红[1] 彭寨玉[1] 

机构地区：[1]中山医科大学寄生虫学教研室,广州510080

出　　处：《中国人兽共患病杂志》2002年第2期44-46,共3页Chinese Journal of Zoonoses

基　　金：国家自然科学基金 (NO 3 0 0 70 683 );国家教育部博士点基金 (NO 2 0 0 0 45 );广东省自然科学基金研究团队项目资助

摘　　要：目的　观察日本血吸虫未知基因SjDad1的DNA免疫保护动物实验效果 ,探讨其作为疫苗候选分子的可能性。方法　构建SjDad1的真核表达载体 pEGFP -N3 -SjDad1,两次质粒DNA免疫BALB/c小鼠后给予小鼠日本血吸虫尾蚴攻击感染 ,设置生理盐水对照组 (NS)和pEGFP -N3 对照组 ,攻击感染后 4 2天处死小鼠 ,计算减虫率和减卵率。结果　双酶切和PCR反应以及测序结果证实重组真核表达载体 pEGFP -N3 -SjDad1构建成功 ,DNA免疫动物的保护实验证明 pEGFP -N3 -SjDad1与生理盐水组相比对小鼠没有显著的减虫作用 (P >0 0 5 ) ,减卵效果具有显著性意义 (P <0 0 1) ,减卵率是6 5 74 %。结论　日本血吸虫 pEGFP -N3 -SjDad1有抗血吸虫生殖作用 ,具有疫苗研究开发价值。Aim To observe protection effect of BALB/c mouse against Schistosoma japonicum infection through DNA immunization of Schistosoma japonicum novel gene Sj Dad1 Methods First construct eukaryotic expression plasmid pEGFP-N 3-Sj Dad1;Then BALB/c mice were immunized by naked plasmid pEGFP-N 3-Sj Dad1.NS group and plasmid pEGFP-N 3 group were set up as control The mice were charged by Schistosoma japonicum cercaria after twice immunization;finally they were killed and statistical analysis was conducted Results The recombinant plasmid pEGFP-N 3-Sj Dad1 was constructed successfully,It was verified by double restriction,PCR reaction and sequencing Statistical analysis of animal experiment showed:in obtaining adult worms,no significant difference exists between pEGFP-N 3-Sj Dad1 group,pEGFP-N 3 group and NS group(P>0 05);In obtaining eggs per gram of liver,significant difference exists between pEGFP-N 3-Sj Dad1 group and NS group(P<0 01),egg reduction rate is 65 74 % Conclusions The novel gene Sj Dad1 is anti-reproductive,it is a possible candidate vaccine molecule

关 键 词：日本血吸虫 抗凋亡因子1 DNA免疫 动物实验 免疫保护 

分 类 号：R383.24[医药卫生—医学寄生虫学]