白介素1受体拮抗剂抑制视网膜色素上皮细胞炎前因子的表达  

Interleukine 1 receptor antigonist inhibits proinflammatory factor production by human retinal pigment epithelial cells in vitro

作　　者：宋宗明[1] 惠延年[1] 瞿佳[2] 王雨生[1] 马吉献[1] 

机构地区：[1]第四军医大学西京医院眼科,西安710032 [2]温州医学院眼视光学院

出　　处：《中华眼底病杂志》2002年第1期49-51,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：国家自然科学基金资助项目 (39970 780 )

摘　　要：目的　观察视网膜色素上皮 (retinal pigment epithelium,RPE)细胞白细胞介素 6 (inter-leukine 6 ,IL - 6 )和白细胞介素 8(interleukine 8,IL - 8)的表达及白细胞介素 1受体拮抗剂 (interleukine 1receptor antigonist,IL- 1ra)对 RPE细胞 IL- 6和 IL- 8表达的影响。　方法　培养的人 RPE细胞分为对照组和 IL - 1ra组 (IL - 1ra,1、10、10 0 ng/ ml) ,用酶联免疫吸附、免疫组织化学和原位杂交等方法观察 RPE细胞 IL- 6和 IL- 8的表达。　结果　对照组 RPE细胞培养上清液中 IL- 6、IL- 8分别为 2 0 0 0、5 0 0 0 pg/ ml。与对照组比较 ,IL - 1ra组 IL - 6、IL - 8分别为 30 0 pg/ ml(t=8.0 11,P <0 .0 1)、4 5 0 pg/ ml(t=11.4 4 6 ,P<0 .0 1)。经白细胞介素 1β(interleukine1β,IL- 1β)刺激后 RPE细胞 IL- 6和 IL- 8m RNA表达原位杂交表现为细胞浆中浓淡不一的蓝色着色 ,IL - 1ra作用组细胞染色较淡。　结论　在 IL - 1β刺激下 ,培养的人 RPE细胞可以表达 IL- 6、IL- 8的 m RNA和蛋白质 ,IL- 1ra能有效抑制其 IL- 6、IL- 8mObjective To investigate production of interleukine 6 (IL 6) and interleukine 8 (IL 8) by retinal pigment epithelial (RPE) cells and its inhibition by interleukine 1 receptor antigonist(IL 1ra). Methods Cultured human RPE cells was treated with interleukine 1 β (IL 1β, 10 ng/ml) and/or IL 1ra ( IL 1ra, 1、10、100 ng/ml). IL 6 and IL 8 mRNA and protein expression were detected by ELISA, immunohistochemistry and in situ hybridization (ISH) assay. Results IL 6 and IL 8 in conditioned media of RPE cells in controls was 2 000 pg/ml and 5 000 pg/ml respectively after stimulation of IL 1β for 8 h. IL 1ra (100 ng/ml) significantly inhibited IL 6 (300 pg/ml, t=8.011, P<0.01 ) and IL 8 (450 pg/ml, t=11.446, P<0.01 ) production compared with the controls. In situ hybridization displayed that there was a marked reduction in mRNA expression of IL 6 and IL 8 in IL 1ra treated group compared with controls. Conclusion The production of IL 6 and IL 8 can be significantly reduced by IL 1ra in cultured human RPE cells after stimulation of IL 1β.

关 键 词：视网膜色素上皮细胞 白细胞介素1受体拮抗剂 增生性玻璃体视网膜病变 炎前因子 

分 类 号：R774.1[医药卫生—眼科]