子宫颈癌细胞亚克隆株细胞周期相关基因的研究  

Study on expression of cell cycle-related genes in subclonal cell lines of human cervical carcinoma

作　　者：陈葳[1] 李旭[1] 付伟[1] 杨玉琮 陈丽宏[2] 程小丽[1] 

机构地区：[1]西安交通大学第一医院实验医学中心,710061 [2]陕西省人民医院妇产科

出　　处：《中华妇产科杂志》2002年第3期168-170,W002,共4页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨不同生长特性的人宫颈癌细胞亚克隆株CS0 3和CS0 7与其细胞周期相关基因的关系。方法　采用流式细胞仪对两株细胞进行细胞周期分析 ,用含有 2 34条人类全长细胞周期相关基因的cDNA表达芯片 ,对两个同一来源、生长特性不同的人宫颈癌细胞亚克隆株CS0 3、CS0 7进行细胞周期相关基因的差异表达谱分析。结果　正常生长条件下培养的CS0 3细胞G1期百分比明显较CS0 7细胞高 ,而CS0 7细胞S期百分比则较CS0 3细胞有所增加 ;在无血清培养条件下CS0 3细胞在4 8h后凋亡 (亚G1期 )细胞百分比明显高于CS0 7细胞 ,并且随着时间的延长而增加。两个亚克隆细胞株CS0 3、CS0 7有 3个差异表达基因 ,分别为人BN5 1mRNA、人热休克蛋白 90 (hsp90 )mRNA和Mcl 1基因 ,荧光强度的比值分别是 0 4 80、0 4 79和 0 4 90。结论　不同生长特性的宫颈癌细胞亚克隆株与其细胞周期相关基因BN5 1、hsp90和MclObjective To explore the role of expression of cell cycle related genes in cervical carcinoma cell lines Methods A series of expression microarray analysis of two homologous cervical carcinoma subclonal cell lines were initiated by cDNA microarray which represent a set of 234 human cell cycle related genes Results In normal medium, the percent of G 1 phase in CS03 cells was higher than in CS07 cells dramatically, but the percent of S phase in CS07 cells was more than in CS03 cells After cultured 48 h in serum free medium,the percent of apoptosis cells (sub G 1 phase) in CS03 cells was higher than in CS07 cells significantly and increased with time By applying this cDNA microarray, we identified 3 differentially expressed genes in two homologous cell lines, which were BN51, hsp90 and Mcl 1 genes,further identified 3 upregulated genes in CS07 cell line, the ratio of Cy5/Cy3 was 0 480, 0 479 and 0 490 respectively Conclusion Differential expression of BN51, hsp90 and Mcl 1 genes is associated to various growth patterns of human cervical carcinoma cells

关 键 词：寡核苷酸序列分析 基因表达调控 细胞周期 子宫颈癌 亚克隆株 

分 类 号：R737.33[医药卫生—肿瘤]