一氧化氮介导白细胞介素-2对离体大鼠心室乳头肌和培养心肌细胞的抑制作用  

Nitric oxide mediates the inhibitory effects of interleukin-2 on the isolated ventricular papillary muscle and cultured neonatal rat cardiomyocyte

作　　者：王琳琳[1] 林国华[1] 陈莹莹[1] 沈岳良[1] 夏强[1] 

机构地区：[1]浙江大学医学院,浙江杭州310031

出　　处：《浙江大学学报（医学版）》2002年第1期19-22,共4页Journal of Zhejiang University(Medical Sciences)

基　　金：浙江省自然科学基金青年人才专项资金;浙江大学医学院中青年科研启动基金

摘　　要：目的 :研究白细胞介素 - 2的离体心肌 /细胞作用及其机制。方法 :采用离体灌流右心室乳头肌和培养乳鼠心肌细胞模型 ,观察重组人白细胞介素 - 2 (hr IL- 2 )对乳头肌平均最大收缩力、收缩间期、舒张间期和培养心肌细胞搏动频率的影响。结果 :1hr IL- 2浓度依赖性地降低离体大鼠心室乳头肌的收缩力 ,高浓度 hr IL - 2缩短收缩间期 ,延长舒张间期 ;2 hr IL- 2减少离体培养乳鼠心肌细胞的搏动频率 ;3L - NAME预处理完全取消 hr IL- 2对离体大鼠心室乳头肌收缩力和乳鼠心肌细胞搏动频率的作用 ;4热失活的 hr IL - 2失去对离体乳头肌和培养心肌细胞的抑制作用。结论 :IL- 2对离体心肌Objective: To explore the effects and mechanism of interleukin 2 on the isolated rat ventricular papillary muscle and cultured cardiomyocyte. Methods: The isolated rat papillary muscle and cultured neonatal rat cardiomyocyte were used to determine the effects on contractile properties and spontaneous beating. Results: IL 2 inhibited the contractile force of isolated rat papillary muscle and reduced the spontaneous beating rate of cultured neonatal rat cardiomyocytes in a dose dependent manner. IL 2 at high concentration reduced the systolic duration and prolonged the diastolic duration. Pretreatment with L NAME, an inhibitor of nitric oxide synthase, abolished the effects of IL 2. Heat inactivated IL 2 had no effect on papillary muscles and cardiomyocytes. Conclusion: Inhibitory effects of IL 2 on the contraction of isolated papillary muscle and spontaneous beating rate of cultured neonatal cardiomyocyte are mediated by nitric oxide.

关 键 词：白细胞介素-2 一氧化氮 一氧化氮合酶 生理学 心肌细胞学 乳头肌 大鼠 

分 类 号：R392[医药卫生—免疫学]