从噬菌体抗体库中淘选人绒毛膜促性腺激素的单链抗体  

Selection of Anti-hCG ScFv from Murine Phage Antibody Library

作　　者：路小龙[1] 张凤英[1] 郭振泉[1] 黄仪秀[1] 朱圣庚[1] 

机构地区：[1]北京大学生命科学学院,北京100871

出　　处：《北京大学学报（自然科学版）》2002年第2期283-285,共3页Acta Scientiarum Naturalium Universitatis Pekinensis

摘　　要：由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。用ELISA方法进行检测 ,从结合力最强的克隆中分离单链抗体基因 ,插入经改造的高效表达载体pET 2 1a(+ )中 ,转化大肠杆菌 ,诱导表达。从培养基中可检测到可溶性抗人绒毛膜促性腺激素的单链抗体。The murine phage antibody library was amplified and then panned by human chorionic gonadotropin (hCG) for four rounds.The last round enriched phage clones were used to reinfect E coli TG1 and 15 separate colonies were assayed by ELISA.The recombinant phages which were rescued from colony 12# showed the highest affinity to immobilized hCG.The Scfv gene was inserted into the reconstructed pET\|21a(+) and expressed in E coli BL21(DE3).The dot blot hybridization verified that the ScFv was secreted to the medium.

关 键 词：噬菌体抗体库 人绒毛膜促性腺激素 单链抗体 ELISA方法 基因工程 抗体克隆 

分 类 号：R392[医药卫生—免疫学] Q78[医药卫生—基础医学]