非损伤性取样的蚕类DNA提取及检测  

The Noninvasive DNA Extraction and Detection of Silkworms

作　　者：吴春旭[1] 李伟[1] 桂慕燕[1] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005

出　　处：《厦门大学学报（自然科学版）》2002年第2期261-264,共4页Journal of Xiamen University：Natural Science

基　　金：国家自然科学基金资助项目 (39870 410 )

摘　　要：对家蚕和蓖麻蚕的蚕蜕和蛾翅分别采用SDS裂解法和动物组织基因组试剂盒提取法提取DNA ,并与常规方法以蚕蛹提取的DNA一起进行RAPD扩增图谱比较、分析 。To obtain DNA noninvasively from the silkworms Bombyx mori and Philosamia Cynthia ricini, we collected the sloughs of fifth instar and the wings of moth, and then extracted DNA by SDS and the DNA extraction kit of animal tissue and purified them. These DNA samples were analyzed by RAPD with 8 arbitrary primes. The results indicated that all the DNA samples had good quality, and that the RAPD pattern of the DNA samples from the silkworms Bombyx mori and Philosamia Cynthia ricini were respectively almost identical.

关 键 词：蚕 DNA非损伤性提取 RAPD 非损伤性取样 DNA提取 DNA检测 分子生物学 

分 类 号：S881.2[农业科学—特种经济动物饲养] Q781[农业科学—畜牧兽医]