新生期注射谷氨酸单钠对大鼠其脑钙结合素表达的影响  

The Effects of Injection of MSG in Newborn Stage of the Calbindin-28kD-expression in the Brain of the Rat

作　　者：金凤霞[1] 刘英发[1] 史玉兰[1] 白文忠[1] 

机构地区：[1]河北师范大学生命科学学院,河北石家庄050016

出　　处：《河北师范大学学报（自然科学版）》2002年第2期196-197,共2页Journal of Hebei Normal University：Natural Science

基　　金：河北省自然科学基金资助项目 ( 3992 0 3)

摘　　要：为探讨谷氨酸单钠 ( monosodium glutamate,MSG)对脑钙结合素 ( calbindin-2 8k D,CB)表达的影响 ,选用新生 SD大鼠 2 9只 ,分为实验组和对照组 ,实验组设 3g/kg和 1 g/kg2个剂量组 ,生后第 3～ 9天经腹腔注射 MSG,对照组设实验对照 (注射生理盐水 1 2 m L/kg)和空白对照 ,满 1 0周龄时 4 %甲醛灌注固定 ,取脑做冰冻冠状切片进行钙结合素的免疫组化反应 .计数各组大鼠丘脑、下丘脑、海马及大脑基底核等脑区钙结合素阳性神经元数量 .发现钙结合素能神经元分布数量具有脑区差异 ,且新生期注射谷氨酸单钠可使成年后某些核团的钙结合素阳性神经元增多 ,表明钙结合素能神经元可抗谷氨酸神经毒性 .To investigate the effects of MSG on the calbindin-28kD-expression in the brain of the rat. Twenty-nine newborn SD rats were divided into two experimental groups and one control group.From the third to the ninth day after birth,the experimental rats were injected intraperitoneally with MSG 1 g/kg or 3 g/kg every day and the controls were given normal saline or untreated.After ten weeks,all the rats were perfused with and their brains were cut into sections and stained by using immunocytochemistry of calbindin-28kD(CB).CB-positive neurons in eight fields of the brain were enumerated under light microscopy.The results indicate that the distribution of CB-positive neurons in the brain of the rat is uneven and injection of MSG might increase the CB-expression in some fields of the brain,which suggest that calbindin-28kD positive neurons resist glutamic neurotoxicity.

关 键 词：脑 钙结合素 谷氨酸单钠 大鼠 免疫组化反应 新生期 表达水平 神经元自我保护机制 

分 类 号：Q421[生物学—神经生物学]