鼠源休眠蛋白具有抑制内皮细胞增殖和诱导细胞凋亡的活性(英文)  

Mouse Restin Inhibits Bovine Aortic Endothelial Cell Proliferation and Causes Cell Apoptosis

作　　者：徐韧[1] 辛利[1] 樊懿[2] 孟海蓉 李载平[1] 甘人宝[1] 

机构地区：[1]中国科学院上海生命科学研究院生物化学与细胞生物学研究所,上海200031 [2]上海中医药大学,上海200032

出　　处：《生物化学与生物物理学报》2002年第2期138-142,共5页

摘　　要：内皮抑素 (endostatin)是最近发现的一种具有抑制血管生长的蛋白质。休眠蛋白 (restin)是内皮抑素的同源蛋白质 ,最早由Ramchandran等人发现 ,它来源于胶原蛋白XV的羧端非胶原蛋白结构域 (NC1)。为了研究鼠源休眠蛋白对内皮细胞生长的影响 ,利用RT PCR从鼠肌肉中扩增出它的基因 ,克隆入原核表达载体pQE32。诱导后 ,重组蛋白质以包涵体形式高效表达 ,表达量约占菌体总蛋白质的 6 0 %～ 70 %。重组蛋白质经纯化复性后 ,可以特异地抑制bFGF刺激的牛主动脉内皮细胞的增殖 ,但是休眠蛋白的抑制活性比内皮抑素活性稍低。流式细胞仪检测发现 ,休眠蛋白可以引起内皮细胞的细胞周期的改变 。Restin, a homologous protein of endostatin, was found by Ramchandran et al. It was the C-terminal fragment of type XV collagen. To analysis the inhibition activity of mouse restin on the proliferation of endothelial cells, the cDNA of restin was amplified from the total RNA of the mouse muscle and cloned into the prokaryotic expression plasmid pQE32. The recombinant protein was expressed in inclusion body with a yield about 60%—70% of total protein. After refolding, the purified recombinant protein specifically inhibits bovine aortic endothelial (BAE) cell proliferation stimulated by basic fibroblast growth factor (bFGF) in a dose-dependent manner, but the activity of restin was weaker than that of endostatin. Treatment of BAE cell with recombinant restin caused G 1 arrest and apoptosis in BAE cells.

关 键 词：休眠蛋白 内皮抑素 胶原蛋白XV 血管生长抑制 细胞凋亡 鼠 

分 类 号：Q51[生物学—生物化学] R73－36[医药卫生—肿瘤]