植物性雌激素genistein对豚鼠乳头肌的电生理效应  

Electrophysiological effects of phytoestrogen genistein on guinea pig papillary muscles

作　　者：马韬[1] 范振中[1] 何瑞荣[1] 

机构地区：[1]河北医科大学基础医学研究所生理室,石家庄050017

出　　处：《生理学报》2002年第1期83-87,共5页Acta Physiologica Sinica

摘　　要：应用细胞内微电极技术 ,观察了genistein (GST)对豚鼠乳头肌的电生理效应。结果显示 :( 1)GST ( 10～ 10 0 μmol/L)浓度依赖地缩短正常乳头肌动作电位时程 ;( 2 )对部分去极化乳头肌 ,GST ( 5 0 μmol/L)除缩短动作电位时程外 ,还使动作电位幅值和超射值降低 ,零相最大上升速度减慢 ;( 3 )预先应用一氧化氮合酶抑制剂L NNA( 5mmol/L) ,不影响GST ( 5 0 μmol/L)的电生理效应 ;( 4 )单独应用 17β 雌二醇 (E2 ,5 μmol/L)或GST ( 10 μmol/L)时 ,动作电位各参数无明显变化 ,而预先应用同剂量的GST再加入E2 ,则动作电位时程缩短。结果提示 ,GST可能通过非NO途径抑制Ca2 +内流 ,从而影响豚鼠乳头肌电生理效应 ,并与E2 有加强或协同效应。The cardiac electrophysiological effects of genistein (GST) were examined in guinea pig papillary muscle using intracellular microelectrode technique. The results obtained are as follows. (1) Duration of action potential (APD) in normal papillary muscles was decreased by GST (10～100 μmol/L) in a concentration dependent manner. (2) In partially depolarized papillary muscles, 50 μmol/L GST not only reduced APD, but also decreased the amplitute of action potential, overshoot and maximal velocity of phase 0 depolarization. (3) Pretreatment with N ω nitro L arginine (L NNA, 5 mmol/L) failed to affect the above effects of GST (50 μmol/L)on papillary muscles. (4) 17β estradiol (E 2, 5 μmol/L) or GST (10 μmol/L) alone did not affect action potential, while GST combined with E 2 at the same doses shortened APD significantly. All these results indicate that the effects of GST on papillary muscles are likely due to a decrease of calcium inflow which is not mediated by NO and that GST has a facilitative or synergetic action with E 2.

关 键 词：植物性雌激素 GENISTEIN 乳头肌 电生理 17Β-雌二醇 豚鼠 心血管 

分 类 号：R331[医药卫生—人体生理学]