家蚕抑前胸腺肽类似物的活性鉴定和结构分析  

Activity identification and structure analysis of the analogs of prothoracicostatic peptide of the silkworm, Bombyx mori

作　　者：刘新[1] 华跃进[1] 陈熙[1] 徐步进[1] 田中良明 

机构地区：[1]浙江大学原子核农业科学研究所,杭州310029 [2]日本农业生物资源研究所,筑波3058634

出　　处：《昆虫学报》2002年第1期8-13,共6页Acta Entomologica Sinica

基　　金：国家自然科学基金高科技探索项目 ( 30 0 80 0 2 2 );教育部"高等院校骨干教师资助计划";教育部优秀回国人员科研启动基金;浙江省自然科学基金 ( 30 0 0 38);浙江省科委重点实验室人才培养项目经费;江苏省教委课题;扬州大学校级课题资助

摘　　要：以家蚕Bombyxmori抑前胸腺肽的氨基酸序列作为基础 ,通过氨基酸残基的添加、减少和置换 ,人工合成了一组与家蚕抑前胸腺肽结构类似的多肽。利用家蚕前胸腺体外培养技术 ,结合蜕皮激素放射免疫分析方法 ,鉴定了与抑前胸腺肽结构类似的多肽的生理活性 ,并对它们的活性特征、化学参数、结构和功能、信号传导途径进行了综合的比较和分析。类似物 89980 8的生物学功能与抑前胸腺肽的相同而且活性近似 ;类似物 89980 5和 89980 9对家蚕前胸腺蜕皮激素的生物合成表现出随浓度增加而增加的促进作用 ,而低浓度下几乎不促进 ;89980 3、 89980 4、 89980 6和 89980 7类似物对家蚕前胸腺蜕皮激素的生物合成的促进和抑制作用与它们的浓度有着依赖关系。实验结果表明 ,对抑前胸腺肽的氨基酸序列作任何改变 ,都导致其生理活性的下降。Based on the amino acid sequence of prothoracicostatic peptide of the silkworm, Bombyx mori, a group of analogs of Bom-PTSP were synthesized by chemical modifications, such as adding, removing, exchanging amino acid of the peptide. By using silkworms' prothoracic glands in vitro culture and 3H-ecdysteriod-radioimmunoassay, the biological activities of those analogs were examined, and their activation features, chemical parameters, and the relationship between structure and function of those peptides were compared and analyzed. It was found the analog 899808 had the same biological function and the similar activity as Bom-PTSP. The analogs 899805 and 899809 partially stimulated ecdysteroidogenesis in a dose dependent manner, while 899803, 899804, 899806 and 899807 had both stimulatory and inhibitory effects on ecdysteroidogenesis, depending on concentration. A possible mechanism of biological activity of Bom-PTSP was discussed.

关 键 词：家蚕 抑前胸腺肽类似物 蜕皮激素 多肽 活性鉴定 结构分析 昆虫 

分 类 号：Q966[生物学—昆虫学]