阴沟肠杆菌B8发酵液对植物的促生作用和IAA分析  

Promoting plant growth and IAA analysis of Enterobacter cloacae B8 fermentation liquid

作　　者：徐幼平[1] 臧荣春[1] 陈卫良[2] 娄沂春[2] 

机构地区：[1]浙江大学分析测试中心,浙江杭州310029 [2]浙江大学生物技术研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2001年第3期282-284,共3页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金!(39870 0 34);浙江省分析测试基金资助项目!(99133)

摘　　要：以甜玉米 Sh2和番茄为试验材料 ,研究阴沟肠杆菌 B8发酵液对植物的促生作用 ,并对其所含的主要生理活性物质进行了分析 .结果表明 :以适当浓度的发酵液喷施植株幼苗期的叶面 ,两周后 ,发现其株高与对照相比明显增加 ,根系也较对照发达 . HPL C证实该发酵液中含有植物生长激素吲哚乙酸( IAA) .在最适条件下 ,培养基中添加 0 .2 %色氨酸时 ,发酵液中 IAA含量可达 90 4 .38mg/ L.试验还发现 ,喷施发酵液后的植株内源WT5”BZ]In this experiment,sweet corn Sh2 and tomato were used to study promoting plant growth of Enterobacter cloacae B8 fermentation liquid, and its main biological active substance was also analyzed. The results showed that the growth of the testing plants could be accelerated after spraying with the suitable concentration of the fermentation liquid. Furthermore, the HPLC method confirmed that the main plant hormone in the fermentation liquid was indole 3 acetic acid (IAA). Under optimum conditions, its content reached 904.38 mg/L in liquid medium when adding 0.2% tryptophan. It was also found the level of endogenous IAA in testing plants was increased. [WT5”HZ]

关 键 词：阴沟肠杆菌B8 发酵液 IAA 促生作用 番茄 甜玉米sh2 生理活性研究 植物生长调节作用 

分 类 号：S482.8[农业科学—农药学]