禽传染性支气管炎病毒S1基因植物表达载体的构建  

The construction of plant expression vector of S1 gene of avian infectious branchitis virus

作　　者：吴建祥[1] 周继勇[1] 程丽琴[1] 沈行燕[1] 丁红梅[1] 

机构地区：[1]浙江大学动物预防医学研究所,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2001年第3期293-296,共4页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目!(30 0 70 5 70 )

摘　　要：:以含传染性支气管炎病毒 ( ZJ971毒株 ) S1基因的质粒 PBS为模板 ,根据其序列设计引物进行PCR扩增 ,得到 1.7kb左右的 S1基因产物 ;用 Xbal I和 Bam HI酶切纯化 ,并在 T4 DNA连接酶的作用下 ,定向克隆到植物表达载体 PBI12 1中 ,PCR及酶切鉴定表明 。WT5”BZ]The S1 gene of avian infectious bronchitis virus (ZJ971 strain) was amplified by polymerase chain reaction with special primers containing XbalI and BamHI. The purified PCR product was digested by the restricted enzyme XbalI and BamHI. The digested and purified S1 gene of infectious bronchitis virus was cloned into plant expression vector pBI121. The result shows that pBI121 with S1 gene was successfully transferred into Agrobacterium tume faciens (Aotumefaciens) with methods of PCR and restricted enzymes. [WT5”HZ]

关 键 词：禽传染性支气管炎病毒 S1基因 植物表达载体 质粒PBS 酚切鉴定 三亲交配 定向克隆 

分 类 号：S858.3[农业科学—临床兽医学] Q782[农业科学—兽医学]