人鼻咽与鼻咽癌cDNA阵谱中DNA修复相关基因表达差异的初步研究  

Plerosis of DNA array of normal human nasopharygeal tissue and nasopharygeal ca rcinoma

作　　者：韩为农[1] 李虹[1] 谢鹭[1] 许亮国[1] 张玲[1] 姚开泰[1] 

机构地区：[1]中南大学湘雅医学院教育部癌变和侵袭重点实验室,长沙410078

出　　处：《中华肿瘤杂志》2002年第2期114-117,共4页Chinese Journal of Oncology

基　　金：国家自然科学基金重点资助项目 (3 973 0 2 0 0 );中华医学会基金重点资助项目 (96 65 5 )

摘　　要：目的　用cDNA阵谱比较鼻咽癌组织及正常组织的基因表达谱 ,研究鼻咽癌组织内修复相关基因的表达差异。方法　AtlashumancancercDNAexpressionarray 774 2 1杂交后 ,用AtlasImage1.0 1a分析滤膜杂交结果 ,RT PCR反应验证滤膜杂交结果。结果　在 5 88个肿瘤相关基因中 ,共有134个基因表达上调 ,88个基因表达下调。在有DNA损伤应答、修复、重组相关基因的C区中 ,表达上调的基因有 2 1个 ,与修复相关的有 6个 ;表达下调的有 4 4个 ,与修复相关的则有 12个。结论DNA修复相关基因的改变可能在鼻咽癌病理生理过程中起一定作用。Objective To compare gene expression gene profilie of nasopharyngeal c arcinoma(NPC) tissue with that of normal nasopharyngeal tissues by cDNA array a nd to discuss possible functions of DNA repair related genes in NPC tissue. Methods After hybridization of atlas human cancer cDNA expression array 7742 1 , atlas hybridization results were analyzed by Atlas Image 1.01a software package. Using RT PCR was used to confirm the results. Results Of 63 differentially expressed genes in quadrangle C including DNA damage respon se, repair & recombination related genes, 6 DNA repair related genes were up regulated, 12 were down regulated. Conclusion DNA repair related genes may be involved in patho physiological process of nas opharyngeal carcinoma.

关 键 词：鼻咽肿瘤 cDNA阵谱 DNA修复相关基因 基因表达 鼻咽癌 

分 类 号：R739.63[医药卫生—肿瘤]