苯乙酸对胶质瘤同源盒基因表达的上调作用  

Up regulation of phenylacetate to glioma homeobox gene expression

作　　者：田宇[1] 杨朝华[1] 赵丛海[1] 

机构地区：[1]吉林大学中日联谊医院神经外科,长春130031

出　　处：《中华肿瘤杂志》2002年第2期126-128,共3页Chinese Journal of Oncology

基　　金：国家自然科学基金资助项目 (3 990 0 15 2 );吉林省科委资助项目 (970 5 67 3 )

摘　　要：目的　观察苯乙酸 (PA)诱导分化胶质瘤细胞C6过程中 ,同源盒 (Hox)基因表达的变化。方法　根据引物的不同 ,将已知的 2 2种Hox基因分为P1、P2、P3三组。用逆转录PCR(RT PCR)及图像分析法 ,分组检测应用PA前后Hox基因组在C6细胞中的表达。对应用PA前后表达显著不同的Hox基因组 ,检测其特异Hox基因的表达。结果　C6细胞中 ,P2组基因表达明显弱于P1、P3组。应用PA后 ,P2组基因及P2组中HoxB2基因表达显著增强 (P <0 .0 0 1)。PA在分化诱导胶质瘤过程中 ,对Hox基因mRNA水平的表达有明显上调作用。结论　PA抑制胶质瘤细胞增殖的作用机理与调节胶质瘤细胞转录过程有关。Objective Even though phenylacetate (PA) bas been shown to inhibit the growth and induce differentiation in rat C6 glioma cell line, its mechanisms are still poorly und erstood. This study is aimed to identify which Hox gene is rel ated to glioma and to observe the change in expres sion on mRNA level as treated by phenylasetate. Methods Twenty two kinds of Hox gene were divided into 3 groups according to their prim er sequence. S emiquantitative reverse transcription polymerase chain reaction (RT PCR) was used to investigate the mR NA expression of Hox gene groups and some Hox gene in rat C6 glioma cell line following differentiation induced by PA. The level of Hox gene expression was expressed as ratio expression rate (RER) of Hox gene /β actin according to computer image analysis and the difference between C6 cells and PA treated C6 c ells was analyzed by student t test. Results It was found that Hox genes matching to primers P2 were mildly expressed in C6 c ells and the expression of HoxB2 mRNA was significantly up regulated i n PA treated C6 cells ( P <0.001). ConclusionThe weak expression of HoxB2 may be involved in glioma origin and the mechanisms of PA action are correlated with transcription process in the glioma cells.

关 键 词：苯乙酸 胶质瘤 同源盒基因 基因表达 上调作用 

分 类 号：R730.2[医药卫生—肿瘤]