γ-谷氨酰转移酶mRNA亚型对肝细胞癌变的监测  

The value of γ-glutamyl transpeptidase mRNA typing in monitoring carcinogensis of heptocytes

作　　者：韩国庆[1] 秦成勇[1] 任万华[1] 石军[1] 刘华琳[1] 

机构地区：[1]山东省立医院肝病中心,济南250021

出　　处：《中华内科杂志》2002年第3期160-162,共3页Chinese Journal of Internal Medicine

摘　　要：目的　探讨γ 谷氨酰转移酶 (GGT)mRNA亚型的转化与原发性肝癌 (HCC)发生的关系 ,寻找肝癌早期诊断的新方法。方法　以逆转录聚合酶链反应方法检测正常对照组、非癌肝病组、肝癌组及肝转移癌肝组织及外周血的 3种GGTmRNA亚型 (A、B、C亚型 )。结果　正常肝组织、非癌肝病的肝组织及肝转移癌癌周组织主要的GGTmRNA类型为A亚型 ,肝癌组织、癌旁组织及远癌组织GGTmRNA B亚型的阳性率显著高于正常肝脏及非癌肝病的肝组织 (P <0 .0 5 ) ,肝癌组织GGTmRNA A亚型阳性率明显低于正常对照及非癌肝病组 (P <0 .0 5 )。在 2 6例HCC中有 12例外周血中检出GGTmRNA B亚型 ,甲胎蛋白阴性的 10例HCC中有 5例检出GGTmRNA B亚型。结论　GGTmRNA亚型转化与肝癌发生有密切关系 ,分析GGT基因可望成为监测肝细胞癌变的灵敏方法。Objective To explore the relationship between the alteration in γ glutamyl transpeptidase (GGT) mRNA expression and the development of hepatocellular carcinoma(HCC). Methods Three GGT mRNA types (A, B, C) in normal liver tissue, diseased liver tissue without HCC, cancerous and noncancerous tissues from livers with HCC, noncancerous tissues from livers with metastatic tumor and peripheral blood of all the cases were tested by using RT PCR. Results In normal livers, the main type of GGT mRNA was type A. In cases with liver diseases but not HCC, the proportion of the types of GGT mRNA was nearly the same as that in normal livers. The proportion of type B was significantly higher in both the cancerous and noncancerous tissues of livers with HCC than in livers without HCC ( P <0.05). The expression of type A in cancerous tissue was significantly lower than that in the tissue of livers without HCC ( P <0.05). Among the 26 cases with HCC, GGT mRNA B was found in the peripheral blood of 12. In 10 HCC cases with negative AFP, GGT mRNA B was found in the peripheral blood of 5. Conclusions GGT mRNA expression in the human liver might shift from type A to type B during the development of HCC. Fragment analysis of GGT genes might be a sensitive assay to monitor the cancerous change of hepatic cell.

关 键 词：肝细胞癌 基因表达 GGT基因 Γ-谷氨酰转移酶 MRNA 亚型 

分 类 号：R735.7[医药卫生—肿瘤]