雌激素受体和凋亡相关基因bcl-x及bax在人原发性胆囊癌中表达的临床意义  

The Expression of Apoptosis-Related Gene bcl-x, bax and Estrogen Receptor in Primary Gallbladder Carcinoma and Its Clinical Significance

作　　者：徐宏勇[1] 李开宗[1] 付由池[1] 窦科峰[1] 阎庆国[2] 王映梅[2] 李景梦[3] 

机构地区：[1]第四军医大学西京医院肝胆外科,西安710032 [2]第四军医大学病理学教研室 [3]解放军第451医院,西安710054

出　　处：《中国普外基础与临床杂志》2002年第2期86-88,共3页Chinese Journal of Bases and Clinics In General Surgery

摘　　要：目的　研究凋亡相关基因bcl x、bax和雌激素受体 (ER)在原发性胆囊癌 (PGC)中表达的意义。方法　应用免疫组织化学LDP法检测石蜡包埋的PGC标本中ER和凋亡相关基因bcl x及bax的表达。结果　bcl x、bax和ER在 47例PGC中表达阳性率分别为 72 .3 % (3 4/4 7)、66.0 % (3 1/4 7)和 5 9.6% (2 8/4 7) ,在 15例胆囊不典型增生中表达阳性率分别为 40 .0 % (6/15 )、93 .3 % (14 /15 )和 93 .3 % (14 /15 )。bax和ER在PGC中的表达显著低于胆囊不典型增生(P<0 .0 5 ) ,而bcl x则高于后者 (P<0 .0 5 )。ER和bax的表达在高分化型PGC显著高于中、低分化型 (P<0 .0 5 ) ,而bcl x则低于后者 (P<0 .0 5 ) ;ER和bax的表达在男性PGC患者显著低于女性患者 (P<0 .0 1) ,而bcl x的表达则男性显著高于女性 (P<0 .0 5 ) ;ER表达在肿瘤直径 ≤2cm者显著高于 >2cm者 (P<0 .0 5 ) ,而bcl x及bax的表达则差异均无显著性意义 (P >0 .0 5 )。bcl x、bax和ER的表达在PGC的Nevin临床分期Ⅰ～Ⅲ期和Ⅳ～Ⅴ期间差异无显著性意义 (P >0 .0 5 )。结论　凋亡相关基因ER和bcl x及bax的表达与PGC的分化程度、性别等因素有关 。Objective To study the relationship between the expression of apoptosis related gene bcl x,bax and estrogen receptor (ER) in primary gallbladder carcinoma (PGC) and its clinical significance. Methods Immunohistochemistry of labeled dextran polymer (LDP) with Envision TM system was used to detect ER and gene bcl x and bax. Results The positive rate of bcl x,bax and ER were 72.3%,66.0% and 59.6% in 47 cases with primary gallbladder carcinoma and 40.0%,93.3% and 93.3% in 6 cases with gallbladder adenomahyperplastic. The expression of bax and ER in PGC was significantly lower than that in gallbladder adenomahyperplastic ( P <0.05),the expression of bcl x was significantly higher in PGC than that in the latter ( P <0.05).The expression of bcl x and ER in well differentiated PGC was significantly higher than that in moderately, poorly differentiated PGC ( P <0.05); bax expression in well differentiated PGC was lower. ER and bax expression in male PGC was significantly lower than that in female PGC ( P <0.01), the expression of bcl x in male PGC was higher ( P <0.05).ER was more highly expressed in smaller PGC than in larger one ( P < 0.05) . ER and bax, bcl x were not different between various clinical stages and ages ( P >0.05,respectively). Conclusion The expression ER, apoptosis related gene bcl x and bax have correlation with differentiation and sex in PGC, their levels shows significance in the prognosis of PGC.

关 键 词：原发性胆囊癌 细胞凋亡 BCL-X基因 BAX基因 雌激素受体 

分 类 号：R735.8[医药卫生—肿瘤]