16型人类乳头状瘤病毒对口腔上皮细胞Rb、p16、Cyclin D1表达的影响  

Influence of HPV16 on expression of Rb, p16 and cyclin D1 in oral epithelial cell

作　　者：帕提曼.司地克 张志愿[1] 曹俊[1] 

机构地区：[1]上海第二医科大学口腔医学院口腔颌面外科,200011

出　　处：《中华口腔医学杂志》2002年第2期84-86,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金资助项目 ( 39770 80 2 )

摘　　要：目的　探讨 16型人类乳头状瘤病毒 (humanpapillomaviruses ,HPV)对口腔上皮细胞Rb、p16、CyclinD1表达的影响 ,以明确HPV16在口腔上皮细胞癌变过程中的作用机制。方法　Western印迹检测人永生化口腔上皮细胞HIOEC中HPV16E6、E7、Rb、p16、CyclinD1蛋白表达 ,免疫沉淀 Western印迹偶联检测HIOEC中HPV16E7 Rb复合物形成情况。结果　HIOEC表达HPV16E6、E7蛋白 ;HIOEC出现去磷酸化Rb堆积 ,HPV16E7和去磷酸化Rb形成复合物 ;p16、CyclinD1蛋白表达无改变。结论HPV16E7在HPV16诱导的口腔上皮细胞永生化过程中起了重要作用。Objective To investigate the role of HPV16E6 and E7 during the transformation of oral epithelial cells. Methods An human immortalized oral epithelial cell line (HIOEC) was established by transfecting HPV16E6,E7 open reading frames using recombinant retroviral system plxsn to human normal oral epithelial cells. Expression of HPV16E6, E7, Rb, P16 and Cycin D1 were analyzed by Western blot in HIOEC and human normal oral epithelial cells. Formation of complex of HPV16E7 and Rb were analyzed by Immunoprecipitation western blot. Human normal oral epithelial cells and the oral epithelial cells transfected with plxsn were used as control groups. Results HIOEC expressed HPV16 E6 and E7; HIOEC expressed both hyperphosphorylated and underphosphorylated Rb while oral epithelial cells in two control groups only expressed hyperphosphorylated Rb. HPV16 E7 formed complex with underphosphorylated Rb; the level of P16 and Cyclin D1 had no remarkable change.Conclusions HPV16E7 plays an important role in the immortalization of oral epithelial cells induced by HPV16.

关 键 词：人乳头状瘤病毒 口腔上皮细胞 永生化 口腔粘膜 HPV p16 RB CYCLIND1 

分 类 号：R739.8[医药卫生—肿瘤]