狗牙周缺损处移植自体骨髓干细胞的骨化实验观察  

The study of calcification of autogenous bone marrow stem cell transplantion on alveolar bone defect in dogs

作　　者：欧龙[1] 刘宏伟[1] 王东胜[1] 罗芸[1] 马良[1] 袁志萍[1] 

机构地区：[1]解放军总医院口腔科,北京100853

出　　处：《中华口腔医学杂志》2002年第2期132-134,T002-T005,共7页Chinese Journal of Stomatology

基　　金：国家自然科学基金 ( 39770 798);国家教委留学回国人员科研启动基金 [外教司留 ( 1997) 436号 ]资助项目

摘　　要：目的　对应用自体骨髓干细胞移植的动物骨化实验的观察进行评价。方法　将 6只成年狗的 36颗牙 ,分为实验组和对照组 (各 18颗牙 )。在人工制造的牙周缺损中进行体外培养的自体骨髓干细胞移植 ,结合引导组织再生 (guidedtissueregeneration ,GTR)方法 (实验组 )和单纯GTR方法(对照组 )。 6周后对切片行牙周组织学观察。结果　实验组新生牙槽骨骨化效果明显好于对照组。实验组建立了正常钙化骨结构 ,Masson染色为红色 ;对照组虽然建立了正常骨结构 ,但钙化程度极低 ,基本为胶原性骨结构 ,Masson染色为蓝绿色。结论　应用自体骨髓干细胞移植结合聚四氟乙烯膜引导牙周组织再生可促进牙周组织的再生、加快正常骨组织结构的建立 ,并缩短修复再生时间。Objective To evaluate the effect of calcification of autogenous bone marrow stem cell transplantation in periodontal tissue regeneration. Methods Bone marrow stem cells derived from the same dog were cultured with α MEM. 1×10 7 cells of first passage were allowed to attach to the collagen membrane for 24 hours.The membrane cells were transplanted into periodontal defect in the same dog. Then the defects were covered with e pTFE membranes. The defects covered only with e pTFE without membrane cells were served as control. Eighteen teeth of 6 dogs for every group were studied. The dogs were sacrificed after 6 weeks. Results The results showed that new bone formation in test group was significantly higher than that of control group. The calcification of new bone in test group was better than control group.Conclusions The results suggested that autogenous bone marrow stem cell transplantation with guided tissue regeneration technique could enhance periodontal tissue regeneration and could form new bone tissue fast and could shorten times of periodontal tissue regeneration in dogs.

关 键 词：细胞移植 干细胞 引导组织再生 狗 牙周缺损 自体移植 

分 类 号：R780.2[医药卫生—口腔医学]