缓激肽B_2受体拮抗剂对致敏豚鼠模型咳嗽反应性的影响  

The effect of bradykinin B_2 receptor antagonist on cough reactivity in a sensitized guinea pig model

作　　者：邱忠民[1] 刘琦[2] 郭德华[1] 傅向春 

机构地区：[1]江西中医学院附属医院呼吸病研究所,南昌330006 [2]日本金汊大学医学部第三内科

出　　处：《中华结核和呼吸杂志》2002年第2期74-77,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

摘　　要：目的　研究缓激肽受体B2 拮抗剂FR1736 5 7对卵蛋白致敏和激发豚鼠咳嗽反应性的影响。方法　正常和卵蛋白致敏豚鼠各 40只 ,卵蛋白雾化吸入激发。 2 4h后 ,再各自分成对照组、小剂量FR1736 5 7组、中剂量FR1736 5 7组和大剂量FR1736 5 7组。每组 10只 ,分别腹腔注射生理盐水、FR1736 5 7溶液 0 0 3mg/kg、0 3mg/kg和 3mg/kg ,观察吸入辣椒素溶液诱导的咳嗽反应。用非侵入性方法测量正常对照组、致敏对照组和致敏中剂量FR1736 5 7组豚鼠的特异性气道阻力。结果　不同剂量的FR1736 5 7不影响正常豚鼠的咳嗽反应和气道阻力。致敏对照组豚鼠吸入 10 -4 mol/L辣椒素溶液诱导的咳嗽次数和特异性气道阻力分别为 [(2 1 7± 3 0 )次 /3min]和 [(9 4± 0 5 )cmH2 O/s]与正常对照组 [(8 3± 1 4)次 /3min ,(7 9± 0 9)cmH2 O/s]比较 ,差异有显著性 (P <0 0 5 ) ,中剂量FR1736 5 7能抑制这些改变 ,咳嗽次数和特异性气道阻力分别为 [(12 2± 1 3)次 /3min ,(7 5± 0 9)cmH2 O/s],两组比较差异有显著性 (P <0 0 5 )。结论　缓激肽B2 受体拮抗剂抑制致敏豚鼠卵蛋白激发后增高的咳嗽反应和气道阻力。因此 ,缓激肽可能是嗜酸细胞性气道炎症所致咳嗽的重要介质。Objective To investigate the effect of bradykinin B 2 receptor antagonist FR173657 on cough response in guinea pigs sensitized and challenged with ovalbumin Methods 40 normal and 40 sensitized guinea pigs were challenged with the aerosol of ovalbumin 24 hours later, 10 animals from the normal group and 10 from the sensitized group were intraperitoneally injected either with saline or with 0 03 mg/kg, 0 3 mg/kg and 3 mg/kg of FR173657 respectively, and then cough response to inhaled capsaicin was measured Specific airway resistance was recorded with a noninvasive technique only in normal, sensitized and FR173657(0 3 mg/kg)-treated sensitized guinea pigs Results FR173657 did not influence cough response and airway resistance in normal guinea pigs Compared with normal animals, sensitized guinea pigs presented an increased cough frequency and specific airway resistance [(21 7±3 0)times/3 min vs (8 3±1 4)times/3 min, (9 4±0 5)cm H 2O/s vs (7 9±0 9)cm H 2O/s], ( P <0 05) after inhalation of 10 -4 mol/L capsaicin The cough frequency and specific airway resistance were decreased to [(12 2±1 3)times/1 min and (7 5±0 9) cm H 2O/s] after administration of 0 3 mg/kg of FR173657 ( P <0 05) Conclusions Bradykinin B 2 receptor antagonist inhibited increased cough response and airway resistance in guinea pigs sensitized and challenged with ovalbumin Bradykinin may be an important mediator in cough associated with eosinophilic airway inflammation

关 键 词：咳嗽变异型哮喘 缓激肽B2受体拮抗剂 咳嗽反应性 辣椒素 实验研究 

分 类 号：R562.25[医药卫生—呼吸系统]