脾虚证大鼠各脑区和血清亮氨酸-脑啡肽的变化  

CONTENT CHANGES OF LEUCINE-ENKEPHALIN IN BRAIN TISSUE AND SERUM IN RAT MODEL OF SPLEEN-ASTHENIA

作　　者：李刚[1] 梁红娟[1] 张贺龙[1] 夏天[2] 

机构地区：[1]中国人民解放军第四军医大学唐都医院,陕西西安710038 [2]中国人民解放军第四军医大学西京医院中医科

出　　处：《安徽中医学院学报》2002年第2期37-39,共3页Journal of Anhui Traditional Chinese Medical College

基　　金：国家自然科学基金资助课题 (392 70 86 4 )

摘　　要：目的 :研究各脑区和血清亮氨酸 脑啡肽 (L EK)含量的变化。方法 :成年SD大鼠 32只随机分为 4组 ,每组 8只 ,即正常组、脾虚组、治疗组、自复组。采用放射免疫分析法 ,测定各组大鼠额叶皮质、下丘脑、垂体及血清L EK的含量。结果 :①脾虚组、自复组大鼠额叶皮质、下丘脑、垂体L EK含量均较正常组、治疗组高 ,有显著性差异 (P <0 .0 1) ;而血清L EK含量均较正常组、治疗组低 ,有显著性差异 (P <0 .0 1)。②大鼠额叶皮质L EK含量均较下丘脑、垂体L EK含量低 ,有显著性差异 (P <0 .0 5 )。结论 :L EK参与脾虚证的病理过程 ,四君子汤通过调节内源性阿片肽 ,发挥健脾益气作用。Objective:To find out the changes of leucine enkephalin(L EK) levels in brain and serum in rat model of spleen asthenia. Method: Rhubarb decoction was used to develop adult SD rat model of spleen asthenia.32 rats were averagely divided into four groups at random,namely,control group, spleen asthenia group,treatment group by Sijunzi decoction, spontaneous rehabilitation group.The radioimmunoassay was applied to measure the L EK levels of rat brain and serum.Result:①In spleen asthenia group and spontaneous rehabilitation group, L EK levels of brain regions were higher than those of control group and spontaneous rehabilitation group (P<0.01);And L EK serum levels were lower than those of control group and spontaneous rehabilitation group (P<0.01); ②L EK levels of frontal cortex were lower than those of hypothalamic, pituitary(P<0.05). Conclusion: L EK is probably involved in the pathophysiology of spleen asthenia ,and Sijunzi decoction may take effect by regulating endogenous opioid peptides.

关 键 词：脾虚证 脑啡肽 亮氨酸 四君子汤 

分 类 号：R228[医药卫生—中医基础理论] R256.3[医药卫生—中医学]