Caspase活化的细胞凋亡在非小细胞肺癌组织中的表达  

Apoptosis with Active Caspase in Human Non-small Cell Lung Cancer

作　　者：卢晓晔[1] 覃莉[1] 黄中新[1] 黄丹[1] 

机构地区：[1]暨南大学医学院,510632

出　　处：《实用癌症杂志》2002年第2期122-124,共3页The Practical Journal of Cancer

基　　金：国家自然科学基金资助项目 (39770 30 0 ) ;国务院侨办基金资助项目

摘　　要：目的　观察非小细胞肺癌 (NSCLC)组织中Caspase活化的癌细胞凋亡与临床病理特征的关系。 方法　对手术切除的NSCLC组织标本 5 0例 (其中鳞癌 2 4例 ,腺癌 2 6例 ) ,采用Caspase特异的单克隆抗体—M 30CytoDEATH免疫组化染色方法显示凋亡细胞 ,计算凋亡指数 (AI)。结果　 2 8.0 % ( 14 /5 0 )的肺癌组织标本中可见不同程度的M 30表达 ,其中肺鳞癌组织的M 30阳性表达率 ( 41.7% )明显高于肺腺癌 ( 15 .3% ) ,有显著性差异 (P <0 .0 5 )。肺鳞癌组织的AI亦高于腺癌 ,有显著性差异 (P <0 .0 5 )。低分化NSCLC组织的AI高于中、高分化的NSCLC组织 ,有显著性差异 (P <0 .0 5 )。低分化鳞癌组织的AI明显高于中、高分化的鳞癌 ,有显著性差异 (P <0 .0 5 )。M 30的表达与其它临床病理学特征无相关性。结论　非小细胞肺癌组织中存在Cas pase活化的自发性癌细胞凋亡机制 。Objective To observe the correlation of apoptosis with active caspase to the clinic characters of human non small cell lung cancer (NSCLC).Methods M30CytoDEATH,an antibody specific for the human Caspase family,was used to display apoptosis cells in 50 NSCLC tissues.The apoptosis index(AI) was calculated.Results The expression of M30 in squamous carcinomas(41.7%) was higher than in adenocarcinomas(15.3%) and the difference was significant.Apoptosis index (AI) in squamous carcinomas was also higher than in adenocarcinomas and the difference was significant.AI in the poorly differentiated carcinomas was significantly higher than in the middle and well differentiated carcinomas.Conclusion Spontaneous apoptosis cells mainly occur in the tissues of squamous carcinoma and poorly differentiated carcinoma.

关 键 词：非小细胞肺癌 半胱氨酸蛋白酶 细胞凋亡 凋亡指数 免疫组织化学 Caspase活化 

分 类 号：R734.2[医药卫生—肿瘤]